Study on the Efficacy of Huazhuo Jiedu Huoxue Tongluo Formula Combined with Tongdu Tiaoshen Acupuncture in the Treatment of Acute Cerebral Infarction and Its Effect on NLRP3 Signaling Pathway
Objective:To investigate the efficacy of Huazhuo Jiedu Huoxue Tongluo Formula combined with Tongdu Tiaoshen Acupuncture in the treatment of acute cerebral infarction(ACI),and its effect on neurological function and the inflammasome signaling pathway of NOD-like receptor thermoprotein domain-related protein 3(NLRP3)in ACI patients.Methods:A total of 80 patients with ACI admitted to our hospital were enrolled and divided into two groups by random number table.The control group was treated with Huazhuo Jiedu Huoxue Ton-gluo Formula,and the observation group was treated with Huazhuo Jiedu Huoxue Tongluo Formula combined with Tongdu Tiaoshen Acupuncture.The differences in efficacy,neurological function,degree of disability,mRNA expres-sion of molecules related to NLRP3 inflammasome signaling pathway,inflammatory factors and oxidative stress be-tween the two groups were observed.Results:The total effective rate of treatment in the observation group was higher than that in the control group(P＜0.05).After treatment,the scores of TCM syndrome scores,the National Institutes of Health Stroke Scale(NIHSS)and modified RANKIN scale(mRS score)scores,the expression of NL-RP3 mRNA,apoptosis-related spot protein(ASC)mRNA,cysteine protease-1(Caspase-1)mRNA in peripheral blood mononuclear cells,serum tumor necrosis factor-α(TN F-α),interleukin(IL)-6,the level of malondialdehyde(MDA)decreased compared with those before treatment(P＜0.05),and the level of superoxide dismutase(SOD)increased compared with that before treatment(P＜0.05).After treatment,the TCM syndrome score,NIHSS and mRS scores,the expression of NLRP3 mRNA,ASC mRNA and Caspase-1 mRNA in peripheral blood mononuclear cells,the serum levels of TNF-α,IL-6 and MDA were lower in the observation groups than those in the control group(P＜0.05),and the levels of SOD were higher than those in the control group(P＜0.05).Conclusion:Hua-zhuo Jiedu Huoxue Tongluo Formula combined with Tongdu Tiaoshen Acupuncture can more effectively inhibit the NLRP3 inflammasome signaling pathway,alleviate inflammation and oxidative stress,improve neurological func-tion,reduce disability,and improve clinical efficacy.
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