Effects of Gegen Decoction on Inflammatory and Immune Indexes TNFR1,NF-κB,JNK in Mice Infected with Influenza A Virus
Objective:To explore the mechanism of anti-influenza viral pneumonia injury of Gegen Decoction based on the mouse mod-el of viral pneumonia caused by PR8 infection.Methods:A total of 32 female SPF ICR mice were randomly divided into the blank group,model group,oseltamivir group and Gegen Decoction group,with 8 mice in each group.A mouse model of viral pneumonia caused by PR8 strain was established by nasal drops.Lung index and inhibition rate of lung index were calculated,and pathological changes of lung tissues were observed by light microscopy.The expression of inflammatory cytokines IL-6,ICAM1,VCAM1 and inflammatory im-mune proteins TNFR1,NF-κB and JNK1 were detected by ELISA and Western Blot.Results:Compared with model group,oseltamivir group and Gegen Decoction group could reduce lung index(P＜0.001),and the inhibition effect of Gegen Decoction group on lung index was better than oseltamivir group.Pathological results showed that compared with model group,Gegen Decoction group could significantly reduce bronchial inflammatory reaction exudation,alveolar,pulmonary interstitial and red blood cell exudation.ELISA results showed that compared with model group,the expressions of IL-6,ICAM1 and VCAM1 in oseltamivir group and Gegen Decoction group were signifi-cantly decreased(P＜0.001).Compared with oseltamivir group,the content of IL-6 in Gegen Decoction group was decreased(P＜0.01).Western Blot results showed that compared with model group,oseltamivir group and Gegen Decoction group could decrease the expres-sions of TNFR1 and JNK1 proteins(P＜0.05 or P＜0.01),Gegen Decoction could reduce the expression of NF-κB protein(P＜0.05).Con-clusion:Gegen Decoction can reduce the expression of IL-6,ICAM1,VCAM1,TNFR1,NF-κB,JNK and other inflammatory factors and inflammatory immune proteins in the lung tissue of mice infected with influenza A virus,regulate inflammation and immune disorders,and provide basic theoretical basis for the clinical application of Zhang Zhongjing's classic pre-scription.
Influenza A virus PR8PneumoniaGegen DecoctionCytokinesInflammatory immune mediatorsPathological injuryMice
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