Effect of Tongqiao Huoxue Decoction on Cerebral Vasospasm and Oxidative Stress in Rats after Subarachnoid Hemorrhage
Objective:To observe the Effect of Tongqiao Huoxue Decoction on cerebral vasospasm(CVS)and ox-idative stress in rats after subarachnoid hemorrhage(SAH).Methods:A total of 72 male SD rats were randomly divided into group A(sham operation group),group B(model group),group C(nimodipine group:4.2 mg/kg),group D(low-dose Tongqiao Huoxue Decoction group:6.3 g/kg),group E(high-dose Tongqiao Huoxue Decoction group:12.6 g/kg),and group F(Tongqiao Huoxue Decoction combined with nimodipine group:Tongqiao Huoxue Decoction 12.6 g/kg+nimodipine 4.2 mg/kg).A rat model of SAH was made using the double-injection method into the cisterna magna,and the rats were intervened with the medication by gavage for 14 consecutive days,while the sham operation group and the model group were given an equal volume of physiological saline by gavage.The neurologic function scores of the rats in each group were assessed;Hematoxylin-Eosin(HE)staining was used to observe the morphological changes of the basilar artery,and the thickness and diameter of the basilar artery wall were measured;the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),nitric oxide(NO),and endothelin-1(ET-1)in brain tissue were detected by enzyme-linked immunosor-bent assay(ELISA).Results:Compared with group A,the neurologic function scores of rats in group B were sig-nificantly decreased,the diameter of the basilar artery lumen was reduced,and the wall thickness increased;the levels of SOD,GSH-PX,and NO in brain tissue were significantly decreased,while the content of MDA and the level of ET-1 were significantly increased(P＜0.01).Compared with group B,the neurologic function scores of rats in groups C,D,E,and F were improved,the diameter of the basilar artery lumen increased,and the wall thick-ness decreased;the levels of SOD,GSH-PX,and NO in brain tissue were elevated,and the content of MDA and the level of ET-1 were reduced(P＜0.05).Compared with group D,the neurologic function scores of rats in groups E and F were improved,the diameter of the basilar artery lumen increased,and the wall thickness de-creased;the levels of SOD,GSH-PX,and NO in brain tissue were elevated,and the content of MDA and the level of ET-1 were reduced.Compared with group C,the neurologic function scores of rats in group F were improved,the diameter of the basilar artery lumen increased,and the wall thickness decreased;the levels of SOD,GSH-PX,and NO in brain tissue were elevated,and the content of MDA and the level of ET-1 were reduced(P＜0.05).Conclusion:Tongqiao Huoxue Decoction can inhibit oxidative stress reactions in rats after SAH,alleviate CVS,and improve neurologic dysfunction and brain damage after SAH in rats.
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