Effects of Qingfei Decoction on Cell Apoptosis and Bcl-2 and Bax Expression in Rats with Acute Respiratory Distress Syndrome through PI3K/Akt Signaling Pathway
Objective:To investigate the involvement of Qingfei Decoction in the process of cell apoptosis in rats with acute respiratory distress syndrome(ARDS)and the mechanism of regulating the PI3K/Akt signaling path-way.Methods:40 SPF level rats were randomly divided into a blank group,a model group,a Qingfei Decoction group,and an inhibitor group,with 10 rats in each group.One and a half hours before modeling,the inhibitor group was injected intraperitoneally with 1 mg/kg of wortmannin.Except for the blank group,the 30 rats were all given tracheal instillation of 3 mg/kg lipopolysaccharide(LPS)100 μL for modeling;each group was kept for obser-vation for 6 hours before starting drug treatment.The blank group and model group were given 12 mL/kg of dis-tilled water by gavage,while the Qingfei Decoction group and inhibitor group were given 12 mL/kg of Qingfei De-coction by gavage;each group was administered once in the morning and once in the evening for 7 consecutive days.After intervention,the apoptosis of lung tissue cells and the expression of PI3K/Akt signaling pathway relat-ed proteins,Bax,and Bcl-2 genes in each group were compared.Results:The expressions of Bcl-2,p-Akt,and p-Gsk-3β in the lung tissue of rats in the model group were significantly lower than those in the blank group,and the expression of Bax and the rate of TUNEL positive cells in lung tissue were significantly increased(P＜0.05).The expressions of Bcl-2,p-Akt,and p-Gsk-3β in the lung tissue of rats in the inhibitor group were significantly lower than those in the model group,and the expression of Bax and the rate of TUNEL-positive cells in the lung tissue were significantly higher(P＜0.05).The expression of Bax in the lung tissue and the rate of TUNEL-posi-tive cells in the lung tissue of the Qingfei Decoction group were significantly lower than those in the model group and the inhibitor group,and the expressions of Bcl-2,p-Akt,and p-Gsk-3β were significantly higher than those in the model group and the inhibitor group(P＜0.05).Conclusion:Qingfei Decoction may participate in the pro-cess of lung tissue cell apoptosis through the PI3K/Akt signaling pathway in the treatment of ARDS.
NETL
NSTL
万方数据
