Investigating the Mechanism of Berberine for Treating Active Ulcerative Colitis Based on GEO Chip Data and Molecular Docking
Objective:To investigate the potential mechanism of berberine in the treatment of ulcerative colitis(UC)through GEO chip data and molecular docking.Methods:The molecular targets of berberine were obtained by Pharm Mapper server;the UC related chip data in GEO database were retrieved,and the differential genes were analyzed by R language;the differential genes between berberine molecular target and UC were enriched and ana-lyzed;the intersection of berberine molecular target and UC differential genes was taken as the key gene,and the potential gene proteins were found by Cytoscape software;the binding of berberine to key genes and potential gene proteins of UC was analyzed by molecular docking technology.Results:A total of 255 molecular targets of berber-ine were screened;based on the analysis of GSE107499 chip in GEO database,860 differential genes were screened,including 545 up-regulated genes and 315 down-regulated genes;the functional annotation of berberine target go was mainly concentrated in the biological processes such as the regulation of transcription factors,DNA transcription and the regulation of tyrosine kinase activity;comparing the enrichment analysis of UC differential gene and berberine target KEGG,it was speculated that berberine treatment of UC was closely related to chemo-kine signaling pathway,T cell receptor signaling pathway and PPAR signaling pathway;through the topological analysis of the key gene network of berberine in the treatment of UC,11 potential gene proteins of berberine in the treatment of UC were selected.The molecular docking verification showed that the ligand and receptor had good binding activity.Conclusion:Berberine exerts its therapeutic effect on UC through multiple targets and pathways,and its mechanism may be related to regulation of immunity and inflammation.
Ulcerative colitisBerberineGEO data miningMolecular dockingMechanism
NETL
NSTL
万方数据
