Effect of Leonurine on Inflammatory Response in Rats with Acute Respiratory Distress Syndrome by Regulating the IRE1A/TXNIP/NLRP3 Signaling Pathway
Objective:To investigate the effect of leonurine on inflammatory response in rats with acute respira-tory distress syndrome(ARDS)by regulating the IRE1α/TXNIP/NLRP3 signaling pathway.Methods:Rats were randomly separated into CK group,Model group,leonurine low-dose(L)group,leonurine high-dose(H)group,and leonurine-H+HY-N2485(NLRP3 activator)group.The lung function of rats was tested.Blood gas analyzer was used to detect partial pressure of oxygen(PaO2)and partial pressure of carbon dioxide(PaCO2).ELISA was ap-plied to detect the levels of IL-10,IL-6,IL-1β,and TNF-α in alveolar lavage fluid.The dry wet ratio of lung tis-sue was detected.The reagent kit was applied to detect MDA content,SOD and CAT activities in lung tissue.HE staining was applied to observe the pathological morphology of rat lung tissue.Western blotting was applied to de-tect the expression of IRE1α,TXNIP,NLRP3,Caspase-1,and Cleaved Caspase-3 proteins in lung tissue.Re-sults:Compared with the CK group,the lung tissue morphology in the Model group was obviously damaged,the levels of PEF,FVC,and FEV0.3/FVC,PaO2,OI,level of IL-10 in alveolar lavage fluid,and activities of CAT and SOD in lung tissue reduced,the level of PaCO2,levels of IL-1β,IL-6,TNF-α in bronchoalveolar lavage fluid,pathological score of lung tissue,content of MDA in lung tissue,and protein expression levels of IRE1α,TXNIP,NLRP3,Caspase-1,and Cleaved Caspase-3 increased(P＜0.05).Compared with the Model group,the lung tissue damage in rats in the leonurine-L and leonurine-H groups was obviously reduced,the levels of PEF,FVC,and FEV0.3/FVC,PaO2,OI,level of IL-10 in alveolar lavage fluid,and activities of CAT and SOD in lung tissue in-creased,the level of PaCO2,levels of IL-1β,IL-6,TNF-α in bronchoalveolar lavage fluid,pathological score of lung tissue,content of MDA in lung tissue,and protein expression levels of IRE1α,TXNIP,NLRP3,Caspase-1,and Cleaved Caspase-3 decreased(P＜0.05).HY-N2485 was able to weaken the improvement effect of leonurine on ARDS rats(P＜0.05).Conclusion:Leonurine can reduce inflammation and oxidative stress,alleviate lung tis-sue damage,and improve lung function in ARDS rats,which may be related to the inhibition of the IRE1α/TXNIP/NLRP3 signaling pathway.
NETL
NSTL
万方数据
