The Protective Effect and Mechanism of Xingnao Enema Fluid on Brain Injury in Mice with Sepsis
Objective:To investigate the protective effect and possible mechanism of Xingnao Enema Fluid on brain injury in mice with sepsis.Methods:50 mice were randomly divided into blank group,model group,blocker group,Xingnao Enema Fluid low and high dose group.Mice were constructed by intraperitoneal injection of LPS to construct a sepsis model.After modeling,the blocker group was given the blocker Nec-1s 2 mg/kg intraperitone-ally for 7 days,once a day;the low and high dose groups of Xingnao Enema Fluid were given 2.5 g/kg and 5.0 g/kg continuous enema for 7 days,once a day;after the intervention,the neurological reflex score was performed,se-rum and brain tissues were collected,and the brain histopathological changes,brain injury markers(NSE,S100β)and inflammatory factors(TNF-α,IL-6)levels,and necroptosis signaling pathway protein expression were ana-lyzed in each group.Results:Compared with the blank group,the neurological reflex score of the model group de-creased,the brain histopathological changes were severe,the levels of brain injury markers(NSE.S100β),serum and brain inflammatory factors(TNF-α,IL-6)increased,and the expression of p-RIPK1,p-RIPK3,p-MLKL and NF-κB P65 proteins in brain tissue increased(P<0.05).Compared with the model group,the pathological chang-es of brain tissue in high-dose Xingnao Enema Fluid group and blocker group were reduced,the levels of brain in-jury markers(NSE,S100β),serum and brain inflammatory factors(TNF-α,IL-6)decreased,and the expression of p-RIPK1,p-RIPK3,p-MLKL and NF-κB P65 proteins in brain tissue was down-regulated(P<0.05).Compared with the low-dose Xingnao Enema Fluid group,the neurological reflex score of the high-dose group was increased,the brain histopathological changes were reduced,the levels of brain injury markers(NSE,S100β),serum and brain inflammatory factors(TNF-α,IL-6)decreased,and the expression of p-RIPK1,p-RIPK3,p-MLKL and NF-κB P65 proteins in brain tissue was down-regulated(P<0.05).Conclusion:Xingnao Enema Fluid has a protec-tive effect on brain injury in mice with sepsis,and its effect is positively correlated with the dose of Xingnao Ene-ma Fluid,and its mechanism of action may be related to its inhibition of RIPK1/RIPK3/MLKL signaling pathway activation,inhibition of NF-κB activation,and inhibition of inflammatory response.