Regulation Effect of Dachaihu Decoction on Inflammatory Response in CCl4-Induced Acute Liver Injury in Rats
Objective:To investigate the protective effect of Dachaihu Decoction on acute liver injury.Meth-ods:Thirty-six male SD rats were randomly divided into normal(NOM)group,model(CCl4)group,ursodeoxycho-lic acid(UDCA)group,and low(DCH-L),medium(DCH-M),high(DCH-H)dose groups of Dachaihu Decoction,with six rats in each group.Liver function indicators were detected by ELISA;serum inflammatory factors and oxi-dative stress indicators of all groups were examined using an automatic biochemical analyzer;pathological changes in all groups were observed by HE and Sirius Red staining;MIP-2 and MIP-1β mRNA expressions were detected by real-time fluorescence PCR;macrophage inflammatory protein-2(MIP-2)protein expression was detected by Western blotting(WB).Results:Compared with the NOM group,the levels of alkaline phosphatase(ALP),alanine transaminase(ALT),aspartate transaminase(AST),γ-glutamyl transpeptidase(γ-GT),direct bilirubin(DBIL),thio-barbituric acid(TBA),and total bilirubin(T-BIL)in the CCI4 group were significantly increased(P＜0.01),while interleukin-6(IL-6),transforming growth factor-β(TGF-β),and tumor necrosis factor-α(TNF-α)levels were al-so elevated(P＜0.01).Superoxide dismutase(SOD)and glutathione(GSH)levels were significantly decreased,and MDA levels were significantly increased(P＜0.01).The levels of MIP-2 and MIP-1β mRNA were significantly increased(P＜0.01),and MIP-2 protein expression was significantly enhanced(P＜0.01).Histological examination showed hepatocyte enlargement accompanied by varying degrees of inflammatory cell infiltration and necrosis,bal-loon-like or fibrous changes were visible;Sirius Red staining showed obvious collagen fiber proliferation and in-creased collagen staining area.Compared with the CCl4 group,UDCA,DCH-L,DCH-M,and DCH-H groups could reverse these indicators and pathological changes,with the high-dose group showing better effects.Conclusion:Dachaihu Decoction can improve liver function indicators and pathological changes in CCL4-induced rats,reduce the levels of inflammatory factors,and inhibit the expression of chemokines MIP-1β and MIP-2.Its potential mechanism is through inhibition of the inflammatory response,thereby exerting a protective effect against acute liv-er injury in CCl4-induced rats.
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