Osthole Inhibited TGF-β1-induced Lung Fibroblast Proliferation and Collagen Synthesis by Down-regulating miR-143-3p
Objective:To investigate the effects of osthole on mouse lung fibroblasts MLF proliferation and col-lagen synthesis and its possible molecular mechanism.Methods:MLF were cultured in vitro and divided into con-trol group,TGF-β1 group,low dose osthole group,medium dose osthole group,high dose osthole group,anti-miR-NC group,anti-miR-143-3p group,high dose osthole+miR-NC group,high dose osthole+miR-143-3p group.RT-qPCR was used to detect the expressions of miR-143-3p.MTT was used to detect cell activity.Western blotting was used to detect protein expression.Results:Compared with the control group,the expression level of miR-143-3p,proliferation and collagen synthesis ability of MLF in TGF-β1 group were significantly increased,and the ex-pression levels of CyclinD1,PCNA,Col Ⅰ and Col Ⅲ were significantly increased(P＜0.05).Compared with TGF-β1 group,medium and high doses of osthole significantly decreased the expression level of miR-143-3p,prolifera-tion and collagen synthesis ability of MLF,and the expression levels of CyclinD1,PCNA,Col Ⅰ and ColⅢ were significantly decreased(P＜0.05).Downregulation of miR-143-3p significantly decreased the proliferation and col-lagen synthesis ability of MLF,and the expression levels of CyclinD1,PCNA,Col Ⅰ and ColⅢ were significantly decreased(P＜0.05).The up-regulation of miR-143-3p reversed the effects of osthole on MLF proliferation and collagen synthesis(P＜0.05).Conclusion:Osthole may inhibit the proliferation and collagen synthesis of mouse lung fibroblasts MLF by down-regulating the expression of miR-143-3p.
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