Experimental Study on the Mechanism of Pingchuan Granules in Inhibiting Airway Remodeling in Asthmatic Mice through Regulation of the IL-33/FBXL19/ST2 Signaling Pathway
Objective:To observe the effects of Pingchuan granules on the IL-33/FBXL19/ST2 signaling path-way and airway remodeling in asthmatic mice.Methods:30 female BALB/C mice aged 6～8 weeks were randomly divided into three groups:the blank group,the model group,and the experimental group.The asthma model was induced using ovalbumin combined with aluminum hydroxide.The mice in the experimental group were treated with Pingchuan granules.After treatment,the mice were sacrificed,and histopathological changes were observed using HE and Masson staining.The level of IL-33 in the bronchoalveolar lavage fluid was detected by ELISA.The positive expression area of ST2 protein was measured by immunohistochemistry.The expression of FBXL19 and ST2r proteins was detected by western blotting.The relative expression levels of IL-4,IL-5,and IL-13 genes were determined by Real-time PCR.Results:Histopathological examination of lung tissue showed that,compared with the model group,the number of inflammatory cells in the Pingchuan granules group was reduced,and the de-position of smooth muscle layer and airway collagen was alleviated.ELISA results showed that the level of IL-33 in the Pingchuan granules group was significantly lower than that in the model group(P＜0.05).Immunohistochem-ical results showed that the positive expression area of ST2 protein in the lung tissue of asthmatic mice treated with Pingchuan granules was significantly lower than that in the model group(P＜0.05).Western blotting results showed that the expression level of FBXL19 protein in the Pingchuan granules group was significantly higher than that in the model group(P＜0.05),while the expression level of ST2 protein was significantly lower(P＜0.05).Re-al-time PCR results showed that the relative expression levels of IL-4,IL-5,and IL-13 genes in the lung tissue of the Pingchuan granules group were significantly lower than those in the model group(P＜0.05).Conclusion:Pingchuan granules can inhibit airway remodeling in asthmatic mice by regulating the activation of the IL-33/FBXL19/ST2 signaling pathway.This may be one of the mechanisms by which Pingchuan granules improve airway remodeling in asthma.
NETL
NSTL
万方数据
