Effect of Yijing Decoction on Retinal miRNA Expression in Rats with Early Diabetic Retinopathy
OBJECTIVE To investigate the impact of Yijing Decoction on microRNA(miRNA)expression in the retinal tissues of rats with early diabetic retinopathy(DR)and identify the target miRNA for Yijing Decoction intervention in early DR.METHODS A total of 20 male SD rats aged two months were used to establish a diabetic rat model with streptozotocin and randomly divided into the model group(MG)and Yijing Decoction group(YJG).Another group of conventionally fed rats served as the control group(CG).YJG rats were orally administered Yijing Decoction at 12.50 g/kg daily,while CG and MG rats were orally administered an equal volume of 0.9%sodium chloride injection.The intervention lasted for 16 weeks.After completing the administration,the body weight of the rats was recorded,and the retinas were removed from both eyes.Total RNA was extracted,and a small RNA library was constructed by PCR amplification for high-throughput sequencing.Differential miRNAs were predicted for target genes,and gene ontology(GO)and pathway analyses were performed on target genes.Differential miRNAs with significant fold changes were selected for PCR validation.RESULTS(1)Known miRNA differences:A total of 644 known miRNAs were screened,with 59 differential miRNAs identified.Compared to the CG group,the MG group had 35 upregulated miRNAs and six downregulated miRNAs,while the YJG group had 20 upregulated miRNAs and fourdownregulated miRNAs.Compared to the MG group,the YJG group had six upregulated miRNAs and 13 downregulated miRNAs,the differences were statistically significant(all P<0.05).Among them,one upregulated miRNA and 11 downregulated miRNAs were commonly differentially expressed in the three groups after Yijing Decoction intervention in diabetic rats.(2)Newly discovered miRNA differences:A total of 242 newly discovered miRNAs were screened,with 105 differential miRNAs identified.Compared to the CG group,the MG group had 50 upregulated miRNAs and 15 downregulated miRNAs,while the YJG group had 55 upregulated miRNAs and 20 downregulated miRNAs.Compared to the MG group,the YJG group had 16 upregulated miRNAs and six downregulated miRNAs,and the differences were statistically significant(all P<0.05).(3)Biological functions and pathways of differential miRNAs:Differential genes were mainly involved in the transcription regulation of various proteins in biological processes.The pathways associated with target genes were mainly tumor-related signaling pathways and lipid metabolism-related pathways.(4)PCR validation:In the MG group,the expression of miR-673-3p in the retina was lower than that in the CG group,and the expression of the remaining 11 miRNAs was higher than that in the CG group.In the YJG group,the expression of miR-673-3p and miR-23b-5p in the retina was higher than that in the MG group,and the expression of the remaining 10 miRNAs was lower than that in the MG group,and the differences were statistically significant(all P<0.05).Except for miR-23b-5p,the other 11 miRNAs were consistent with the results of the above RNA detection.CONCLUSIONS The miR-673,miR-1,miR-133,miR-214,miR-23b,miR-31a,miR-451,etc.,may be target miRNAs for Yijing Decoction intervention in early microvascular damage in DR.
NETL
NSTL
万方数据
维普
