Relationship between serum miR-199a-3p level and DR in patients with T2DM
OBJECTIVE To investigate the correlation between serum miR-199a-3p levels and diabetic retinopathy(DR)in patients with type 2 diabetes mellitus(T2DM).METHODS A total of 81 patients(162 eyes)diagnosed with T2DM at Eye Hospital,China Academy of Chinese Medical Sciences from September 2021 to August 2022 were enrolled in the study.The patients were divided into two groups based on the presence of DR:The DR group(51 cases,102 eyes)and the NDR group(30 cases,60 eyes).Additionally,30 healthy individuals(60 eyes)who underwent physical examinations during the same period were selected as the control group.Venous blood samples(5 mL)were collected from the control group during physical examinations and from the DR and NDR groups upon admission.The relative expression levels of serum miR-199a-3p in the three groups were detected using quantitative real-time polymerase chain reaction(qRT-PCR),and serum biochemical indicators were also measured.The correlation between serum miR-199a-3p levels and the occurrence of DR was analyzed,and the predictive value for DR was evaluated.RESULTS(1)Biochemical indicators:Significant differences were observed in the levels of fasting plasma glucose(FPG),glycated hemoglobin(HbA1c),alanine aminotransferase(ALT),aspartate aminotransferase(AST),and triglycerides(TG)among the three groups(FFPG=88.218,FHbA1c=92.815,FALT=28.522,FAST=19.647,FTG=27.680,all P=0.000).The NDR group had significantly higher levels of FPG,HbA1c,and ALT compared to the control group(tFPG=10.302,tHbA1c=9.977,both P=0.000;tALT=2.924,P=0.015).However,there were no significant differences in AST and TG levels between the NDR group and the control group(P>0.05).The DR group had significantly higher levels of all biochemical indicators compared to the control group and the NDR group(control group:tFPG=15.123,tHbA1c=15.275,tALT=7.357,tAST=6.649,tTG=7.898,all P=0.000;NDR group:tFPG=3.929,tHbA1c=3.772,both P=0.001;tAST=3.591,P=0.002;tALT=4.410,tTG=4.210,both P=0.000).(2)Serum miR-199a-3p levels:There were significant differences in serum miR-199a-3p levels among the three groups(F=25.100,P=0.000).Both the NDR and DR groups had significantly higher serum miR-199a-3p levels compared to the control group(tNDR=2.147,P=0.035;tDR=6.517,P=0.000).The DR group had significantly higher serum miR-199a-3p levels than the NDR group(t=4.606,P=0.000).(3)Serum miR-199a-3p and biochemical indicator levels in different stages of DR:Patients in the proliferative diabetic retinopathy(PDR)stage had significantly lower serum miR-199a-3p levels compared to those in the non-proliferative diabetic retinopathy(NPDR)stage(t=7.272,P=0.000).Patients in the PDR stage had significantly higher levels of FPG,HbA1c,ALT,AST,and TG compared to those in the NPDR stage(tFPG=4.444,tHbA1c=4.074,tALT=3.818,all P=0.000;tAST=2.172,P=0.035;tTG=3.587,P=0.001).(4)Correlation analysis of serum miR-199a-3p levels with T2DM duration and biochemical indicators in DR patients:Pearson correlation analysis showed that serum miR-199a-3p levels in DR patients were negatively correlated with the duration of T2DM,FPG,HbA1c,ALT,AST,and TG levels(rT2DM=-0.368,P=0.008;rHbA1c=-0.456,P=0.001;rAST=-0.416,P=0.002;rALT=-0.405,P=0.003;rFPG=-0.472,rTG=-0.488,both P=0.000).(5)Correlation analysis of serum miR-199a-3p levels with the occurrence of DR in T2DM patients:The area under the receiver operating characteristic curve(ROC)for predicting the occurrence of DR using serum miR-199a-3p levels in T2DM patients was 0.783,indicating statistical significance(P=0.000).Serum miR-199a-3p levels demonstrated a certain predictive performance for diagnosing DR.The optimal cutoff point of the ROC yielded a Youden index of 0.475,with a sensitivity of 0.867 and a specificity of 0.608.CONCLUSIONS The expression of serum miR-199a-3p in T2DM patients is closely associated with the occurrence of DR and is correlated with the duration of DR,blood glucose levels,and lipid metabolism indicators.Serum miR-199a-3p may serve as a potential biomarker for predicting DR in T2DM patients.
type 2 diabetes mellitusdiabetic retinopathymiR-199a-3p
万方数据
维普
