On the Base of Integrated Network Administration to Explore the TCM Mechanism of Medicines for the Ttreatment of Diabetic Retinopathy
OBJECTIVE To integrate a medication network to explore the mechanisms of Traditional Chinese Medicine(TCM)in the treatment of diabetic retinopathy(DR).METHODS Relevant literature on TCM treatment for DR from CNKI,Wanfang,VIP,CBM,PubMed,MEDLINE,Embase,and Web of Science databases up to October 2022 was included.Statistical analysis was performed using the Ancient and Modern Medical Case Cloud Platform and IBM SPSS Modeler 18.0 to identify core prescriptions for treating DR.The TCMSP,GeneCards,OMIM,and TTD databases were utilized to screen the prescription's components,targets,and DR-related targets to identify core components and targets.Molecular docking was conducted using Autodock and Pymol software to obtain and visualize the binding energy heatmap of ligands and receptors.RESULTS(1)Data analysis and medication network construction:A total of 216 articles were included,yielding 265 TCM prescriptions involving 295 different Chinese medicines with a cumulative frequency of 2,937 occurrences.29 high-frequency Chinese medicines(used≥25 times)were identified.Cluster analysis of these high-frequency Chinese medicines resulted in three combinations.Class Ⅰ,Clearing heat and cooling the blood,replenishing Qi,and invigorating blood.Class Ⅱ,Tonifying the liver and kidney.Class Ⅲ:Activating blood,removing stasis,replenishing Qi,and nourishing Yin.Association rule analysis identified the following high correlation combinations,Poria-Alisma,Moutan Cortex;Poria-Alisma,Moutan Cortex,Cornu;Poria-Alisma,Moutan Cortex,Rehmannia;Poria-Alisma,Moutan Cortex,Dioscorea and Poria-Alisma,Cornus,Dioscorea.The modified Liuwei Dihuang Pill was identified as the core prescription,consisting of Rehmannia,Cornus,Dioscorea,Alisma,Poria,Moutan Cortex,Astragalus,Salvia,Angelic,Panax Notoginseng,and Rhizoma Chuanxiong.(2)Medicine target and protein model construction and analysis:The core prescription was found to contain 114 active components and 1,879 targets.There were 1,385 DR-related targets,with 214 intersecting targets.The"medicine-active component-target"network identified key components acting on DR,including quercetin,kaempferol,23-acetyl-alismol B,and β-sitosterol.The visualized protein-protein interaction(PPI)networks revealed core targets as SRC,STAT3,MAPK1,AKT1,and TP53.Molecular docking was completed for the protein structures of five core targets and the small molecule structures of four core components.CONCLUSIONS The data analysis identified that the modified Liuwei Dihuang Pill has the efficacy of replenishing Qi and nourishing Yin,removing stasis,and stopping bleeding for the treatment of DR.The prescription features multiple components,targets,and pathways,offering research value in promoting systemic glucose homeostasis and improving retinal vascular lesions and other DR-related symptoms.
Traditional Chinese medicinediabetic retinopathydrug mechanisms
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