Simultaneous determination of seven heavy metal elements in human blood and urine by inductively coupled plasma tandem mass spectrometry
Objective To establish a method to simultaneously determinate cobalt,nickel,arsenic,molybdenum,silver,cadmium and lead in blood and urine using inductively coupled plasma tandem mass spectrometry(ICP-MS/MS).Methods The blood samples were diluted 20 times with a mixed solution of nitric acid(0.10%V/V)and Triton X-100(0.02%V/V).The urine samples were diluted 10 times with nitric acid(1.00%V/V).Yttrium-89,rhodium-103,and lutetium-175 were used as internal standards to decrease matrix interference,and either On-Mass mode or Mass-Shift mode was used to decrease mass spectrometry interference,with detection by ICP-MS/MS.Results The linear ranges of the seven elements were 0.100-10.000 µg/L,with the correlation coefficient>0.999 9.The detection limits for the seven elements in the blood and urine were 0.003-0.021 and 0.003-0.031 µg/L,respectively,and the minimum quantification limits were 0.009-0.064 and 0.009-0.094 µg/L,respectively.The recovery rates were 96.64%-102.90%and 96.34%-104.50%for the blood and urine,respectively.The within-run relative standard deviations(RSDs)were 0.32%-2.33%and 0.25%-2.31%,and the between-run RSDs were 1.07%-3.81%and 1.30%-3.62%for blood and urine,respectively.The samples were stable at 4℃for at least 14 days.Conclusion ICP-MS/MS is a simple,sensitive,and accurate method for rapid detection of seven heavy metal elements in the blood and urine of occupational hazard-exposed workers.
Inductively coupled plasma tandem mass spectrometryMass spectrum interferenceBloodUrineHeavy metal element
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