Determination of three forms of mercury in human hair by high performance liquid chromatography-atomic fluorescence spectrometry
Objective To establish a method for the determination of mercury,methylmercury(MeHg)and ethylmercury(EtHg)in the hair of mercury-exposed individuals using high performance liquid chromatography-atomic fluorescence spectrometry.Methods About 0.20 g of hair was mixed with 5.0 mL of hydrochloric acid to a concentration of 5.00 mol/L.After standing for 12 hours,ultrasonic extraction was carried out for 30 minutes at room temperature.Ammonia solution was added dropwise into 2.0 mL supernatant after centrifuge to adjust the pH to 4-5.The solution was diluted to 10.0 mL with pure water after mixed with 0.2 mL of L-cysteine with a mass concentration of 10.0 g/L.The mobile phase consisted of 5.0%methanol+a mass concentration of 0.06 mol/L ammonium acetate+a 0.1%L-cysteine,and was used for separation by a C18 liquid chromatography column,followed by detection using atomic fluorescence spectrometry after UV digestion.Results The linear relationship of mercury,MeHg and EtHg in human hair was good at mass concentrations of 0.0-10.0 µg/L,with all correlation coefficients>0.999 0.The detection limit of mercury,MeHg and EtHg was 0.008,0.008,and 0.010 µg/g,respectively.The within-and between-run relative standard deviations of the three forms of mercury were 1.6%-4.3%and 2.0%-5.6%,respectively,and the recovery rates were 94.0%-101.2%.Conclusion This method is simple,with low detection limit,high sensitivity,and good accuracy,and is suitable for the simultaneous determination of mercury,MeHg and EtHg in the hair of mercury-exposed individuals.
High performance liquid chromatographyAtomic fluorescence spectrometryHuman hairMercuryMethylmercuryEthylmercury
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