Studies on Polysaccharide of Cynomorium songaricum Rupr.
Objective:To study the acidic heteropolysac charides of Cynomorium songaricum.Method:The polysaccharides were purified by Sephadex G-100 and G-150 gel column chromatography.Purity and molecular weight of the polysaccharides were determined by high performance gel permeation chromatography; neutral sugars composition were identified by PC, TLC and GC. Uronic acids were determined by carbazole method.Result:The molecular weight of SYP-A and SYP-B were estimated to be 3.1×105 and 2.8×105 respectively, and neutral sugars were composed of galactose, glucose, arabinose, rhamnose, mannose and ribose. The molar ratio for SYP-A and SYP-B were 5.1∶4.1∶1.6∶1.0∶0.5∶0.3 and 5.2∶4.2∶1.5∶1.0∶0.5∶0.2 respectively, The contents of uronic acids were 10.7% and 10.5% respectively.Conclusion:SYP-A and SYP-B are homogeneous acidic heteropolysaccharides.
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万方数据
维普
