Objective: To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici. Method: The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design. Result: The best extraction condition as follows; 81. 0℃of the extraction temperature,1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best. Conclusion: A model equation that can be used to predict the experiment is established through the response surface method.