鸡内金的分子鉴定研究
Identification of corium stomachium galli by molecular taxonomy
曲萌 1崔继春 2董志恒 1张丽华 1王冰梅 1张吉林 1李才2
作者信息
- 1. 北华大学医学部,吉林,吉林,132013
- 2. 吉林化工二院,吉林,吉林,132001
- 折叠
摘要
目的:建立一种简便、实用、准确的鸡内金药材DNA分子标记鉴定方法,从而为建立鸡内金真伪鉴别方法奠定基础.方法:从Genbank数据库下载家鸡及3种常见伪内金源动物的mtDNA细胞色素b基因序列,经同位置序列比较,遵循引物设计原则选择一个差异点较多的片段,设计PCR引物(引物1,2),并使用该引物分别扩增家鸡、3种伪内金源动物,4种非伪内金源动物的DNA.结果:所设计的引物只扩增家鸡DNA,而不扩增其他动物DNA.结论:该引物具有特异性,可用于鸡内金药材的鉴定.
Abstract
Objective:To establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.Method: Cytb mtDNA sequences of Callus gaUus domestica and three other species of poultry were downloaded from Genbank.Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes.PCR tests were performed with the DNAs extracted from G.gallus domestica,three other poultry species and domestic mammal animals.Result: The specific primers of G.gallus domestica could only amplify the cytb mtDNA of G.gallus domestica.Conclusion: The primers are specific to G.gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.
关键词
鸡内金/PCR扩增/线粒体DNA/种属特异性引物Key words
corium stomachium galli/PCR/mtDNA/species-specific PCR primers引用本文复制引用
基金项目
吉林省教育厅资助项目(吉教合字2006第自52号)
出版年
2009
国家科技期刊平台
NSTL
维普
万方数据