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突托蜡梅叶中挥发油成分及其抑菌活性研究

Chemical composition and antibacterial activity of the essential oil from leaves of Chimonanthus grammatus

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目的:研究突托蜡梅Chimonanthus grammatus 叶中挥发油的化学组成及其抑菌活性.方法:采用气相色谱-质谱联用(GC-MS)技术对水蒸气蒸馏法提取的突托蜡梅叶中挥发油进行成分分析,并采用滤纸片琼脂平板扩散法及肉汤稀释法检测该挥发油对9种常见病原菌的抑菌活性.结果:从挥发油中检出了53种成分,所鉴定成分占挥发油色谱总峰面积的99.99％,其主要成分为(E)-3-(4,8-二甲基-3,7-壬烯基)-呋喃(13.81％)、乙酸冰片酯(12.66％)、1,2,3,6-四甲基-双环[2.2.2]辛-2-烯(7.06％)等；抑菌实验表明突托蜡梅叶中挥发油对受试菌均有不同强度的抑制作用.结论:突托蜡梅叶中挥发油对革兰阴性、阳性菌均具有较强的抑菌活性,抑菌谱较广,且该挥发油含有多种生物活性成分,具有很高的药用价值及开发前景.
The chemical composition of essential oil, which from the leaves of Chimorumthus grammatus obtained by hydrodis-tillation were analyzed by GC-MS, and their possible antibacterial properties were screened. According to the results from GC-MS analysis, fifty-three components comprising 99. 99% of the essential oil were identified. The major components of essential oil were 3-(4, 8-dimethyl-3 ,7-nonadienyl) -(E) -furan ( 13. 81 % ) , bornyl acetate ( 12. 66% ) , and 6,6-dimethyl-3-methylene-bicyclo[3. 1.1] heptane (7. 06% ) , etc. Antibacterial activity of essential oil was employed by two complementary test systems of disc diffusion and MIC/ MBC tests, which showed obviously antibacterial activity against all of the tested bacteria.

Chimonanthus grammatusessential oilGC-MSantibacterial activity

刘易鑫、颜日明、鲁顺保、张志斌、邹峥嵘、朱笃

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江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌330022

江西省林业科学院竹类研究所,江西南昌330032

宜春学院江西省天然药物活性成分研究重点实验室,江西宜春336000

突托蜡梅 挥发油 GC-MS 抑菌活性

国家自然科学基金江西省自然科学基金江西省自然科学基金江西省教育厅科技项目江西省教育厅科技项目

310600902009GZN01022010GZN0113GJ J1039510702

2011

10.4268/cjcmm20112219

中国中药杂志
中国药学会

中国中药杂志

CSTPCDCSCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2011.36(22)
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