Study on preparation of quercetin nanostructured lipid carriers and their physicochemical properties
Objective:To prepare quercetin nanostuctured lipid carriers (QT-NLC),and detect their physicochemical properties.Method:QT-NLC was prepared by emulsification ultrasonic dispersion method,and the optimum prescription was screened out by orthogonal design.Transmission electron microscope was used to observe QT-NLC morphology.Granulometer was applied to deter-mine zeta potential,particle size and distribution.DSC was adopted for phase analysis.Centrifugal ultra-filtration method was used to determine entrapment efficiency.Dialysis method was adopted to detect drug release in vitro of preparations.Result:QT-NLC prepared under optimum conditions was mostly spherical grains,with the average particle size of (175 ±25) nm,which were distributed evenly,and zeta potential was(-23 ±0.3) mY.DSC results indicated that the drug was dispersed in nano-particles in a non-crystalline state,with an entrapment efficiency of (95.43 ±0.23) ％ and a drug-loading capacity of (2.38 ±0.24) ％.The in vitro drug release was 32.2％ in 2 hours,which was followed by a sustained release.Conclusion:Emulsification ultrasonic dispersion method is applicable for preparing QT-NLC,as nano-particles are distributed evenly,with good reliability.This processing technology is safe,reliable and highly reproducible.
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万方数据
维普
