采用多种色谱技术,从黄水茄的全草中分离得到10个化合物,通过MS,NMR等波谱学方法鉴定了化合物的结构,包括5个皂苷类化合物和5个黄酮苷类化合物,分别为anguiviosides XV(1),smilaxchinoside A (2),methylprotodioscin(3),protodioscin(4),solamargine(5),3'4’,5-三羟基-7-甲氧-6-C-β-D-葡萄糖黄酮苷(6),brainoside B (7),camsibriside A(8),kampferol-3-O-(2"-β-D-galactowranosyl)-β-D-glucopyranoside(9),quercetin-3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside(10).化合物1～10为首次从黄水茄中分离得到.运用MTT法进行体外抗肿瘤实验,结果表明化合物4和5均显示一定程度的细胞增殖抑制活性.
Ten compounds,including five steroidal saponins and five flavonol glycosides,were isolated from the whole plant of Solanum coagulans by means of column chromatographies over silica gel,ODS,Sephadex LH-20,and preparative HPLC.Based on analysis of MS and NMR spectroscopic data,their structures were established as anguiviosides XV (1),smilaxchinoside A (2),methylproto-dioscin(3),protodioscin(4),solamargine (5),3',4',5-trihydroxy-7-methoxy-6-C-β-D-glucopyranoside (6),brainoside B (7),camsibriside A (8),kampferol 3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (9),and quercetin-3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside(10).All the compounds were first isolated from this plant.In the in vitro assays,compounds 4 and 5 showed cytotoxic activity against SMCC-7721 and NCI-H460.
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