Molecular identification of hairy antler by analysis of high resolution melting
High resolution melting (HRM),an important technology for genotyping and mutation scanning,has broad prospects in the authenticity of traditional Chinese medicine.This paper selected universal CO Ⅰ primers and used HRM to establish a new method for authenticity of Hairy Antler.PCR was conducted at the annealing temperature of 60 ℃ and 45 cycles.The range of the DNA template concentration,the primer concentration and the Mg2+ ion concentration were further optimized.The results showed that the Tm values of Cervus nippon were (81.96 ±0.07),(84.51 ±0.03) ℃ and Cervus elaphus was(82.58 ±0.13),(85.95 ±0.05) ℃ with 10-100 mg · L-1 DNA template,0.2 μmol · L-1 primer,2.0 mmol · L-1 Mg2+.This method can authenticate of hairy antler and is simple,fast,high-throughput,visualization.
high resolution meltinghairy antlermolecular identification
NETL
NSTL
万方数据
维普
