Simultaneous determination of benzophenones and xanthone in leaves of Aquilaria sinensis by RP-HPLC-UV
This study is to develop a sensitive method by using reversed-phase high performance liquid chromatography coupled with UV detector (HPLC-UV) to simultaneously determine four bioactive compounds,iriflophenone 3-C-β-D-glucoside,iriflophenone 3,5-C-β-D-diglucoside,mangiferin,and iriflophenone 2-O-α-L-rhamnoside in the leaves of Aquilaria sinensis.An Agilent Zorbax SB-C18 column (4.6 mm ×250 mm,5 μm) was used,and the gradient elution was performed with mobile phase of 0.1％ aqueous phosphoric acid and acetonitrile at a flow rate of 1 mL · min-1.The detection wavelength was 280 nm,and the column temperature was 25 ℃.The four marker compounds were well separated with good linearity (R2 ＞0.999 0),precision,stability and repeatability.The recovery rates were in the range of 98.80％-101.39％.For 15 branch of the leaves,the contents of iriflophenone 3-C-β-D-glucoside,iriflophenone 3,5-C-β-D-diglucoside,mangiferin,and iriflophenone 2-O-α-L-rhamnoside were between 0.41-14.48,0.72-3.85,4.30-29.07,0.24-5.06 mg,respectivley.This method is precise,accurate and reliable,which provides an efficient way for the quality control of the leaves of A.sinensis.This will promote the comprehensive usage of this plant.
NETL
NSTL
万方数据
