Studies on genetic diversity of three Tibetan herbs
The genetic diversity of three Tibetan herbs, i.e., Sang-Di, E-Dewa and Ye-Xingba (Tibetan names), was studied based on the field collection, specimen identification and DNA sequence analysis.Swertia hispidicalyx, Gentiana lhassica and Scrophularia dentata, as the original plants of the three Tibetan herbs, were collected and identified.The regions of ITS, matK, rbcL, rpoC1, trnL (UAA) ,psbA-trnH, atpB-rbcL, trnS (GCU)-trnG (UCC), rpl20-rps 12, trnL (UAA)-trnF (GAA) and nadl 2 nd intron were amplified and sequenced.The ITS regions of S.hispidicalyx and S.dentata were cloned and sequenced, and the sequences were classified into different genotypes.All the sequences were analyzed and compared with those of closely related species.Our studies may provide reference for the genetic diversity analysis and molecular identification of the three Tibetan herbs.
NETL
NSTL
万方数据
维普
