基于转录组测序揭示适度干旱胁迫对甘草根基因表达的调控

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NETL
NSTL
万方数据
维普

中文摘要：适度干旱胁迫可促进甘草有效成分的积累,提高药材质量.该研究以栽培6个月的甘草根为材料,设置适度干旱胁迫组(土壤相对含水量40％～ 45％)和对照组(土壤相对含水量70％～75％),分别应用Illumina HiSeq 2000进行转录组双末端测序,分析甘草根响应适度干旱胁迫的差异表达基因.在适度干旱胁迫组和对照组中分别得到80 490 490,82 588 278个Clean reads,经序列拼接和去冗余处理分别得到94 828,305 100个Unigene序列,序列长度的中位数分别为1 007,1 125 nt.差异表达基因分析表明,适度干旱胁迫抑制细胞壁中β-木糖苷酶、天冬酰胺酰内肽酶、GDP-L-岩藻糖合酶等酶的基因表达,可能抑制根细胞的初生壁降解与程序性细胞死亡;促进萜类、黄酮类化合物生物合成的关键酶基因的表达,进而促进甘草有效成分的积累;促进生长素、乙烯、细胞分裂素的生物合成和信号转导,进而促进根的发生和细胞增殖;促进脱落酸、茉莉酸的生物合成和信号转导,进而提高甘草对干旱胁迫的适应能力;抑制赤霉素、油菜素内酯等激素的生物合成和信号转导,进而抑制地上茎的伸长.

外文标题：De novo sequencing and analysis of root transcriptome to reveal regulation of gene expression by moderate drought stress in Glycyrrhiza uralensis

外文摘要：Moderate drought stress has been found to promote the accumulation of active ingredients in Glycyrrhiza uralensis root and hence improve the medicinal quality.In this study,the transcriptomes of 6-month-old moderate drought stressed and control G.uralensis root (the relative water content in soil was 40％-45％ and 70％-75％,respectively) were sequenced using Illumina HiSeq 2000.A total of 80 490 490 and 82 588 278 clean reads,94 828 and 305 100 unigenes with N50 sequence of 1 007 and 1 125 nt were obtained in drought treated and control transcriptome,respectively.Differentially expressed genes analysis revealed that the genes of some cell wall enzymes such asβ-xylosidase,legumain and GDP-L-fucose synthase were down-regulated indicating that moderate drought stress might inhibit the primary cell wall degradation and programmed cell death in root cells.The genes of some key enzymes involved in terpenoid and flavonoid biosynthesis were up-regulated by moderate drought stress might be the reason for the enhancement for the active ingredients accumulation in G.uralensis root.The promotion of the biosynthesis and signal transduction of auxin,ethylene and cytokinins by moderate drought stress might enhance the root formation and cell proliferation.The promotion of the biosynthesis and signal transduction of abscisic acid and jasmonic acid by moderate drought stress might enhance the drought stress tolerance in G.uralensis.The inhibition of the biosynthesis and signal transduction of gibberellin and brassinolide by moderate drought stress might retard the shoot growth in G.uralensis.

外文关键词：

Glycyrrhiza uralensisdrought stresstranscriptome sequencingsecondary metabolismplant hormones

作者：

张春荣、桑雪雨、渠萌、唐晓敏、程轩轩、潘利明、杨全

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作者单位：

广东药学院中药学院,广东广州510006

中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700

关键词：

甘草干旱胁迫转录组测序次级代谢植物激素

基金：

国家自然科学基金中医药行业科研专项中国中医科学院自主选题研究项目中国中医科学院中药资源中心项目广东省中医药局建设中医药强省科研项目

项目编号：

811734882012070022011ZDXK-01KFKT201300520142090

出版年：

2015

DOI：

10.4268/cjcmm20152417

中国中药杂志

中国药学会

中国中药杂志

CSTPCDCSCD北大核心

影响因子：1.718

ISSN：1001-5302

年,卷(期)：2015.40(24)

被引量26
参考文献量2