Screening for differentially-expressed proteins in ovary of primary dysmenorrheal mice with Xiangfu Siwu decoction administration using nano LC-LTQ-Orbitrap-MS/MS
To study the mechanism of Xiangfu Siwu decoction in treating primary dysmenorrhea,differentially-expressed proteins in ovary of primary dysmenorrheal mice with Xiangfu Siwu decoction administration were screened based on proteome technology using nano LC-LTQ-Orbitrap-MS/MS.Estradiol benzoate and oxytocin were used to produce dysmenorrheal mice model.The model mice were orally administrated with Xiangfu Siwu decoction for 3 days,and 1 h after the last administration,the ovary samples were collected.After protein denaturation,reduction,alkylation,desalination and enzymatic hydrolysis,identification was carried out by nano LC-LTQ-Orbitrap-MS/MS technology.The obtained data was processed by using Thermo Proteome discoverer 1.4 software.The differentially-expressed proteins were screened and identified,and their biological information was also analyzed.The significant differentially-expressed protein was checked using Western blot technology in ovary samples.A total of 106 differentially-expressed proteins were identified during the normal,model and administration group.Most of them participate cellular processes.Adherens junction and focal adhesion pathways play a regulatory role in various cell signaling pathways.Protein ADRM1 was validated.Compared to the normal group,it was up-regulated expression in the model group.After administration,the expression of ADRM1 was down-regulated.Through the comparative analysis,a series of differentially-expressed proteins involved in primary dysmenorrheal mice with Xiangfu Siwu decoction administration were obtained.Protein ADRM1 may become a target for Xiangfu Siwu decoction.
NETL
NSTL
万方数据
维普
