首页|基于指标成分与近红外光谱对宁夏野生和栽培甘草的比较鉴别研究

基于指标成分与近红外光谱对宁夏野生和栽培甘草的比较鉴别研究

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比较宁夏乌拉尔甘草(以下均简称甘草)中基于指标成分含量的色谱鉴别和基于近红外光谱的光谱鉴别差异,建立快速有效鉴别宁夏野生和栽培甘草的方法.采用HPLC和紫外光谱法测定9批野生甘草和14批栽培甘草中甘草苷、甘草酸和总黄酮的含量并采集其近红外光谱信息.结果显示,基于指标成分定量的色谱鉴别无法有效鉴别宁夏野生和栽培甘草,近红外光谱结合聚类分析和主成分分析能够直观、快速、有效鉴别宁夏野生和栽培甘草样品,为甘草生长方式的鉴别及应用研究提供有效方法.同时,为甘草的品种、产地及规格等方面的鉴别提供了研究思路.
Comparative identification studies on wild and cultivated Glycyrrhiza uralensis produced Ningxia based on index components and near-infrared spectroscopy
This study is to construct a rapid and effective method for identification of wild and cultivated Glycyrrhiza uralensis (hereinafter referred to as Glycyrrhizae Radix et Rhizoma)from Ningxia by comparison of the difference in chromatography identifica-tion based on index components and near-infrared spectroscopy identification.HPLC and UV methods were used to determine the con-tent of liquiritin,glycyrrhizate and total flavonoids for 9 wild Glycyrrhizae Radix et Rhizoma and 14 cultivated Glycyrrhizae Radix et Rhizoma samples,and the near-infrared spectroscopy was also,collected.The results illustrated that the chromatography identification based on index components could not identify wild and cultivated Glycyrrhizae Radix et Rhizoma from Ningxia,while near-infrared spectroscopy could quickly and effectively achieve it.It provides an effective method for the growth pattern identification and application of Glycyrrhizae Radix et Rhizoma.

Glycyrrhiza uralensis (Glycyrrhizae Radix et Rhizoma)near-infrared spectroscopyindex componentscluster analy-sisprincipal components analysisidentification

狄天云、高晓娟、张霞、赵建军、雍婧姣、马玲、王英华、王汉卿

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宁夏医科大学 药学院,宁夏 银川 750004

宁夏回族自治区药品检验所,宁夏 银川 750004

宁夏回药现代化工程技术研究中心,宁夏 银川 750004

宁夏医科大学 回医药现代化省部共建教育部重点实验室,宁夏 银川 750004

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甘草 近红外 指标成分 聚类分析 主成分分析 鉴别

国家自然科学基金青年项目宁夏回族自治区重点研发项目宁夏自然科学基金

816032272016KJHM46NZ15091

2017

中国中药杂志
中国药学会

中国中药杂志

CSTPCDCSCD北大核心
影响因子:1.718
ISSN:1001-5302
年,卷(期):2017.42(3)
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