首页|多穗柯转录组分析及黄酮类化合物合成相关基因的挖掘

多穗柯转录组分析及黄酮类化合物合成相关基因的挖掘

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多穗柯具有甜味和保健功效,其中黄酮类化物是主要活性物质.为获取多穗柯转录组数据库以及黄酮类化合物生物合成相关基因,该研究用RNA-Seq中的Illumina HiSeq 4000对多穗柯嫩叶进行转录组测序,共获得6 Gb数据,拼接后得到41 043条Unigene,与7个基因数据库进行比对,可归类于51个GO分类中,涉及到237个KEGG标准代谢通路.找到黄酮合成相关基因28条.通过MicroSatallite (MISA)软件分析筛选到18 161个SSR,其中单碱基重复最丰富,有7 346个.此研究得到大量转录本信息,为多穗柯的分子生物学研究提供了宝贵的转录组数据库资源.
Analysis of transicriptomes and exploring flavonoid biosynthetic pathway genes in Lithocarpus polystachyus
The sweet taste and health effect of Lithocarpus polystachyus are mainly related flavonoid.To obtain Lithocarpus transcriptome database and flavonoid biosynthesis-related genes,the RNA-Seq techology (Illumina HiSeq 4000) was used to sequence its transcriptome.Six Gb database was assembled after assembly steps,and 41 043 of L.polystachyus unigenes were obtained.With blasting them with 7 data banks,all unigenes were involved in 51 GO-terms and 237 metabolic pathways.And furthermore 28 genes of the flavonoid biosynthesis-related were found.After using the MicroSatallite,18 161 SSR were obtained,the single-nucleotide-repeated was the richest at 7 346.These data represent abundant messages about transcripts and provide valuable genome data sources in molecular biology of L.polystachyus.

Lithocarpus polystachyustranscriptomehigh-throughput sequencingflavonoids

宋菊、黄剑、李志栋、龙月红、邢朝斌

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华北理工大学生命科学院,河北唐山063000

百色学院农业与食品工程学院,广西百色533000

多穗柯 转录组 高通量测序 黄酮类化合物

国家自然科学基金河北省教育厅科研项目华北理工大学培育基金

31570683QN2014102SP201508

2017

中国中药杂志
中国药学会

中国中药杂志

CSTPCDCSCD北大核心
影响因子:1.718
ISSN:1001-5302
年,卷(期):2017.42(4)
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