首页|天麻硫熏标志物p-hydroxybenzyl hydrogen sulfite的定量分析及稳定性研究

天麻硫熏标志物p-hydroxybenzyl hydrogen sulfite的定量分析及稳定性研究

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特征硫熏标志物研究已成为中药质量控制的有效辅助手段.该研究建立了UPLC-MS/MS测定天麻硫熏产物p-hydroxybenzyl hydrogen sulfite的定量分析方法.应用所建立的方法对37批天麻药材进行筛查,发现27批样品是经过硫熏处理的,硫熏较严重的为云南产区的天麻样品.通过考察不同贮藏时间和加热处理下p-hydroxybenzyl hydrogen sulfite成分的稳定性,发现药材中该成分含量在8个月的贮藏期内以及正常加热处理(50,60,70,80℃)条件下是稳定可靠的.以上研究表明该硫熏产物可作为天麻硫熏标志物,具有灵敏度高、选择性好和稳定性好,为天麻质量控制和硫熏药材筛查提供参考.
Quantitative analysis and stability of p-hydroxybenzyl hydrogen sulfite in sulfur-fumigated Gastrodiae Rhizoma
Studies on the characteristic chemical markers of sulfur fumigation have become an effective auxiliary way for quality control of traditional Chinese medicine.This study established a quantitative analysis method for the determination ofp-hydroxybenzyl hydrogen sulfite (p-HS) in Gastrodiae Rhizoma (GR) based on UPLC-MS/MS.Then,37 batches of GR were screened,and the results showed that 27 batches of them were sulfur-fumigated.Especially,the GR samples in Yunnan producing areas were sulfur-fumigated more seriously.Based on the stability of p-HS after different storage time and heat treatment methods,it was found that the content of p-HS was stable and reliable in the storage period of 8 months and under normal heat treatment (50,60,70,80 ℃) conditions.In conclusion,this study shows a high sensitivity,good selectivity and good stability of p-HS,which can provide reference for the quality control and sulfur fumigation screening of GR.

Gastrodiae Rhizomachemical marker of sulfur fumigationUPLC-MS/MSstabilityquality evaluation

康传志、蒋靖怡、杨婉珍、周利、吕朝耕、李佳兴、王升、周涛、杨野、黄璐琦、郭兰萍

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中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700

贵阳中医学院,贵州贵阳550025

昆明理工大学,云南昆明650093

天麻 硫熏标志物 UPLC-MS/MS 稳定性 质量评价

国家自然科学基金国家自然科学基金国家重点研发计划项目中央本级重大增减支项目发改委标准化项目中国中医科学院博士研究生创新人才培养基金云南省科技项目

81325023814733072017YFC17007012060302ZYBZH-C-HLJ-17CX2017052017ZF014

2018

中国中药杂志
中国药学会

中国中药杂志

CSTPCDCSCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2018.43(2)
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