Screening of bioactive components endowing hawthorn with turbidity-eliminating and lipid-lowering functions and development of quality control method of hawthorn
Hawthorn has the efficacy of eliminating turbidity and lowering the blood lipid level,and it is used for treating hyperlipi-demia in clinic.However,the bioactive components of hawthorn are still unclear.In this study,the spectrum-effect relationship was employed to screen the bioactive components of hawthorn in the treatment of hyperlipidemia,and then the bioactive components screened out were verified in vivo.Furthermore,the quality control method for hawthorn was developed based on liquid chromatogra-phy-mass spectrometry(LC-MS).The hyperlipidemia model of rats was built,and different polar fractions of hawthorn extracts and their combinations were administrated by gavage.The effects of different hawthorn extract fractions on the total cholesterol(TC),tri-glycerides(TG),and low-density lipoprotein-cholesterol(LDL-C)in the serum of model rats were studied.The orthogonal projections to latent structures(OPLS)algorithm was used to establish the spectrum-effect relationship model between the 24 chemical components of hawthorn and the pharmacodynamic indexes,and the bioactive components were screened out and verified in vivo.Finally,10 chemical components of hawthorn,including citric acid and quinic acid,were selected to establish the method for evaluating hawthorn quality based on LC-MS.The results showed that different polar fractions of hawthorn extracts and their combinations regulated the TG,TC,and LDL-C levels in the serum of the model rats.The bioactive components of hawthorn screened by the OPLS model were vitexin-4″-O-glucoside,vitexin-2″-O-rhamnoside,rutin,citric acid,malic acid,and quinic acid.The 10 chemical components of hawthorn,i.e.,citric acid,quinic acid,rutin,gallic acid,vitexin-4″-O-glucoside,vitexin-2″-O-rhamnoside,malic acid,vanillic acid,neochlo-rogenic acid,and fumaric acid were determined,with the average content of 38,11,0.018,0.009 5,0.037,0.017,8.1,0.009 5,0.073,and 0.98 mg·g-1,respectively.This study provided a scientific basis for elucidating the material basis of hawthorn in treating hyperlipidemia and developed a content determination method for evaluating the quality of hawthorn.
hawthornhyperlipidemiaspectrum-effect relationshipcontent determinationquality control
NETL
NSTL
万方数据
维普
