Quality evaluation of salt-fired Eucommiae Cortex based on HPLC fingerprint,multi-component content determination,and chemometrics
This study established an HPLC fingerprint and multi-component content determination method for salt-fired Eucommiae Cortex,and evaluated the quality of salt-fired Eucommiae Cortex from different sources using fingerprint similarity evaluation,cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least square discriminate analysis(OPLS-DA).HPLC was launched on a Cosmosil 5C18-MS-Ⅱ column(4.6 mm×250 mm,5 μm)by gradient elution with a mobile phase of methanol-0.2%phosphoric acid aqueous solution at a flow rate of 1.0 mL·min-1,detection wavelength of 238 nm,column temperature of 30℃,and an injection volume of 10 μL.The results of fingerprint similarity evaluation for 20 batches of salt-fired Eucommiae Cortex indicated that,except for batch S3 with a similarity of 0.893,the similarity of the other 19 batches was of≥0.919,suggesting good similarity.Fourteen common peaks were calibrated and seven common peaks were identified including geniposidic acid.The mass fractions of ge-niposidic acid,chlorogenic acid,geniposide,genipin,pinoresinol diglucoside,liriodendrin,and pinoresinol-4-O-β-D-glucopyranoside were 0.062 0%-0.426 9%,0.024 9%-0.116 5%,0.009 5%-0.052 9%,0.005 5%-0.034 8%,0.115 9%-0.317 8%,0.016 4%-0.108 8%,and 0.026 4%-0.039 8%,respectively.Using CA,PCA,and OPLS-DA,the 20 batches of salt-fired Eucommiae Cortex were classified into three categories.Additionally,through the analysis of variable importance in projection(VIP)under OPLS-DA,two differential quality markers,geniposidic acid and chlorogenic acid,were identified.The established HPLC fingerprint and multi-component content determination method is stable and reliable,providing a reference for quality control of salt-fired Eucommiae Cortex.
NETL
NSTL
万方数据
维普
