摘要
采用大孔树脂、硅胶、RP-C18 柱色谱及HPLC等色谱分离技术从秦艽Gentiana macrophylla的 70%乙醇提取物中分离得到 13 个化合物,分别鉴定为macrophylloside G(1)、macrophylloside D(2)、5-formyl-2,3-dihydroisocoumarin(3)、(+)-medi-carpin(4)、(+)-syringaresinol(5)、liquiritigenin(6)、(3R)-sativanone(7)、(3R)-3′-O-methylviolanone(8)、4,2′,4′-tri-hydroxychalcone(9)、latifolin(10)、gentioxepine(11)、6α-hydroxycyclonerolidol(12)、ethyl linoleate(13).其中化合物1 为1 个新的苯并吡喃糖苷类化合物,化合物 4、6～10、12 和13 均为首次从秦艽和龙胆属中分离得到.化合物1 和2 在10 μmol·L-1浓度下对用D-GalN诱导的AML12 细胞肝损伤模型具有显著的保护活性,其中化合物 2 比同浓度下的水飞蓟宾活性更加显著.
Abstract
Thirteen compounds were isolated and identified from 70%ethanol extract of the roots of Gentiana macrophylla by multi-chromatographic methods,including microporous resin,silica gel,and C18 reversed-phase column chromatography,as well as HPLC as follows:macrophylloside G(1),macrophylloside D(2),5-formyl-2,3-dihydroisocoumarin(3),(+)-medicarpin(4),(+)-syringa-resinol(5),liquiritigenin(6),(3R)-sativanone(7),(3R)-3′-O-methylviolanone(8),4,2′,4′-trihydroxychalcone(9),latifolin(10),gentioxepine(11),6α-hydroxycyclonerolidol(12),and ethyl linoleate(13).Compound 1 was a new benzopyran glycoside.Compounds 4,6-10,12,and 13 were isolated for the first time from Gentiana plants.Compounds 1 and 2 showed promising hepatopro-tective activity against D-GalN-induced AML12 cell damage at the concentration of 10 μmol·L-1,and compound 2 exhibited more sig-nificant activity than silybin at the same concentration.
基金项目
广东省教育厅普通高校新冠肺炎疫情防控专项(2020KZDZX1203)
NETL
NSTL
维普
万方数据