A new benzopyran glycoside from Gentiana macrophylla
Thirteen compounds were isolated and identified from 70%ethanol extract of the roots of Gentiana macrophylla by multi-chromatographic methods,including microporous resin,silica gel,and C18 reversed-phase column chromatography,as well as HPLC as follows:macrophylloside G(1),macrophylloside D(2),5-formyl-2,3-dihydroisocoumarin(3),(+)-medicarpin(4),(+)-syringa-resinol(5),liquiritigenin(6),(3R)-sativanone(7),(3R)-3′-O-methylviolanone(8),4,2′,4′-trihydroxychalcone(9),latifolin(10),gentioxepine(11),6α-hydroxycyclonerolidol(12),and ethyl linoleate(13).Compound 1 was a new benzopyran glycoside.Compounds 4,6-10,12,and 13 were isolated for the first time from Gentiana plants.Compounds 1 and 2 showed promising hepatopro-tective activity against D-GalN-induced AML12 cell damage at the concentration of 10 μmol·L-1,and compound 2 exhibited more sig-nificant activity than silybin at the same concentration.
NETL
NSTL
万方数据
维普
