Characterization and identification of chemical constituents from Schizonepetae Spica based on UHPLC-Q-TOF-MS/MS technique
The chemical constituents of Schizonepetae Spica were qualitatively analyzed by UHPLC-Q-TOF-MS/MS.An Agilent po-roshell 120 SB-C18 column(3.0 mm×100 mm,2.7 μm)was used for gradient elution with 0.1%formic acid water(A)-acetonitrile(B)solution as mobile phase at the flow rate of 0.4 mL·min-1 and column temperature of 45℃.The data were collected by scanning in positive and negative ion modes,and the compounds were identified by comparison of reference materials and PeakView software.Ninety-seven compounds were identified from Schizonepetae Spica,including 28 flavonoids,23 phenolic acids,23 fatty acids,15 ter-penoids,and 8 other compounds.The UHPLC-Q-TOF-MS/MS method established in this study can identify the chemical components of Schizonepetae Spica rapidly,accurately,and comprehensively,and provide a basis for the basic study of pharmacodynamic sub-stances of Schizonepetae Spica.
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万方数据
维普
