外翻肠囊法研究五味清浊散的肠吸收成分及其吸收特征
Intestinal absorption components and absorption characteristics of Wuwei Qingzhuo San by everted intestinal sac models
廖翠平 1董世奇 2刘云璐 1额尼日勒 3高雅 1樊慧蓉 2蔡秋杰 4代金刚 1图雅5
作者信息
- 1. 中国中医科学院 医学实验中心, 北京 100700
- 2. 中国医学科学院 放射医学研究所, 天津 300192
- 3. 内蒙古民族大学 蒙医药学院, 内蒙古 通辽 028000
- 4. 中国中医科学院 临床基础研究所, 北京 100700
- 5. 中国中医科学院 中医药研究发展中心, 北京 100700
- 折叠
摘要
研究五味清浊散在不同肠段的吸收情况,以及其生物碱类成分胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁的吸收特征.通过构建大鼠肠外翻模型,应用UPLC-Q-TOF-MS技术测定五味清浊散在不同肠段透过的化学成分,以及采用UPLC-Q-TRAP-MS技术测定肠吸收液中胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁的含量,计算其吸收参数,并分析其在大鼠不同肠段、不同时间的吸收特征.实验结果显示,在建立的UPLC-Q-TOF-MS条件下,在空肠、回肠、十二指肠和结肠段的肠囊液中分别检测到 27、27、8、6 个吸收成分;且发现五味清浊散中胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁在各肠段均为线性吸收,r均达到0.9 以上,各肠段的吸收量胡椒碱>胡椒新碱>二氢荜茇宁>荜茇宁,但各成分在各个肠段的吸收速率及其机制不尽相同,提示在不同肠段五味清浊散提取液各成分的吸收具有选择性,并不是简单的半透膜渗透过程.
Abstract
This study investigated the absorption profile of Wuwei Qingzhuo San in different intestinal segments and the absorption characteristics of its alkaloids(piperine,piperanine,piperlonguminine,and dihydropiperlonguminine).The everted gut sac model was established,and the chemical components of Wuwei Qingzhuo San in different intestinal segments were detected by UPLC-Q-TOF-MS.The content of piperine,piperanine,piperlonguminine,and dihydropiperlonguminine in intestinal absorption fluid was determined by UPLC-Q-TRAP-MS and the absorption parameters were calculated.The absorption characteristics in different intestinal segments at different time were analyzed.As a result,27,27,8,and 6 absorbent components from Wuwei Qingzhuo San were detected in the intestinal cyst fluid of jejunum,ileum,duodenum,and colon by UPLC-Q-TOF-MS technology,respectively.It was also found that piperine,piperanine,piperlonguminine,and dihydropiperlonguminine from Wuwei Qingzhuo San showed linear absorption in various intestinal segments,with r values exceeding 0.9.In terms of absorption content,the components were ranked as piperine>piperanine>dihydropiperlonguminine>piperlonguminine in various intestinal segments,but the absorption rate and mechanism of each component varied.The results demonstrate that the absorption of the components of Wuwei Qingzhuo San in different intestinal segments is selective and is not a simple semi-permeable membrane permeation process.
关键词
五味清浊散/胡椒碱/胡椒新碱/荜茇宁/二氢荜茇宁/肠外翻模型Key words
Wuwei Qingzhuo San/piperine/piperanine/piperlonguminine/dihydropiperlonguminine/everted intestinal sac model引用本文复制引用
基金项目
国家自然科学基金面上项目(82274212)
中国中医科学院医学实验中心协同创新团队项目(XTCX2021004)
出版年
2024
NETL
NSTL
维普
万方数据