摘要
为明确不同栽培型白花前胡的代谢差异,揭示质量形成机制,为前胡种质挖掘与利用提供参考依据.该研究基于超高效液相色谱-质谱联用技术(UHPLC-MS/MS),对同一产地、同一时期的6种栽培型前胡(YS、H、LZ、LY、LX、Z)进行非靶向代谢组学分析,利用主成分分析、正交偏最小二乘法判别分析、单变量统计分析等方法筛选不同栽培型前胡的差异代谢物,根据质荷比、数据库KEGG、相关文献、相关性分析确定与质量形成相关的潜在标志代谢物,识别代谢途径,分析质量形成过程.结果表明,6种栽培型前胡代谢物存在明显差异,正、负离子模式下,分别得到571、465种差异代谢物.其中,与质量形成相关的潜在标志代谢物22种,涉及苯丙烷生物合成、植物激素的生物合成及苯丙氨酸、酪氨酸和色氨酸生物合成等9种潜在靶向代谢通路.与YS型相比,各栽培型前胡中与苯丙烷生物合成相关的补骨脂素、欧前胡素、鲁望桔内酯下降,7-羟基香豆素、秦皮乙素、二氢欧山芹素、茉莉酸升高;其他代谢途径的2-琥珀酰苯甲酸酯、独活属醇、L-酪氨酸下降,以Z、H型的变化最大.因此,合理调控苯丙烷生物合成途径是栽培型前胡质量提升的关键机制之一,Z、H型具有更优的质量形成代谢过程可用于前胡优质种质的筛选与挖掘.
Abstract
This study aims to reveal the quality formation of different cultivars of Peucedanum praeruptorum based on the metabolic differences and provide a theoretical basis for the development and utilization of this medicinal herb.The non-target metabonomics anal-ysis based on ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)was conducted for six culti-vars(YS,H,LZ,LY,LX,and Z)of P.praeruptorum of the same origin and at the same development stage.The principal compo-nent analysis,orthogonal partial least squares discriminant analysis,and univariate statistical analysis were carried out to screen the differential metabolites of different cultivars.The potential biomarkers associated with quality formation were predicted based on the mass-to-charge ratio,Kyoto Encyclopedia of Genes and Genomes pathway enrichment,information of relevant literature,and correlation analysis.The results showed that metabolites differed significantly among the six cultivars,and 571 and 465 differential metabolites were obtained in the positive and negative ion modes,respectively.From the differential metabolites,22 potential biomarkers related to quality formation were predicted,which involved 9 metabolic pathways,including phenylalanine,tyrosine and tryptophan biosynthesis,biosynthesis of phenylpropanoids,and biosynthesis of plant hormones.Compared with the YS cultivar,other cultivars showed decreased concentrations of psoralen,imperatorin,and luvangetin and increased concentrations of 7-hydroxycoumarine,esculetin,columbianetin,and jasmonic acid,which were involved in the biosynthesis of phenylpropanoids.The concentrations of 2-succinylbenzoate,heraclenol,and L-tyrosine involved in other metabolic pathways decreased,especially in the Z and H cultivars.Therefore,regulating the biosyn-thesis of phenylpropanoids is one of the key mechanisms for improving the cultivar quality of P.praeruptorum.The Z and H cultivars have better quality and metabolic processes than other cultivars and thus can be used for the screening and breeding of high-quality germplasm.
基金项目
重庆市自然科学基金(cstc2021jcyjmsxmX0664)
重庆市技术创新与应用发展重点项目(CSTC2021jscxgksb-N0035)
重庆市科技局市场服务激励项目(22JK46-2312)
维普
万方数据