摘要
对稀花八角枫70％乙醇提取物化学成分进行研究.采用D-101大孔树脂、硅胶、Sephadex LH-20等柱色谱技术对稀花八角枫70％乙醇提取物的化学成分进行分离纯化,从中分离鉴定出19个化合物,分别为4-环己烯-1α,2α,3α-三醇-1-O-β-D-葡萄糖苷(1)、1β,4α,6α,13-tetrahydroxy-eudesm-11(12)-ene(2)、蔗糖(3)、1'-O-benzyl-α-L-rhamnopyranosyl-(1"→6')-β-D-glu-copyranoside(4)、双(2-乙基己基)苯-1,2-二羧酸酯(5)、(Z)-10-二十碳烯酸(6)、di-O-methylcrenatin(7)、甲基-α-D-呋喃果糖苷(8)、β-胡萝卜苷(9)、丁香酸(10)、香草酸(11)、二十八烷醇(12)、isoarborinol(13),2,7-dihydroxy-6-methyl-4-(1-methylethyl)-1-naphthalenecarboxylate(14)、香草醛(15)、松柏醛(16)、9(11)-去氢麦角甾醇过氧化物(17)、5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(18)、β-谷甾醇(19).其中化合物1和2为新化合物,化合物5～11、13、15～18为首次从八角枫属中分离得到.通过脂多糖(LPS)诱导的RAW264.7巨噬细胞炎症模型对化合物1进行了抗炎活性测定,结果显示,化合物1对LPS诱导的RAW264.7细胞NO生成具有一定的抑制作用,其抑制率为54.57％.
Abstract
Chemical constituents of 70％ethanol extract of Alangium chinense subsp.pauciflorum were investigated.The 70％ethanol extract of A.chinense subsp.pauciflorum was isolated and purified by D-101 macroporous resins,silica gel,Sephadex LH-20 and other methods.As a result,nineteen compounds were isolated and identified as 4-cyclohexene-1α,2α,3α-triol-1-O-β-D-glucoside(1),1β,4α,6α,13-tetrahydroxy-eudesm-11(12)-ene(2),sucrose(3),1'-O-benzyl-α-L-rhamnopyranosyl-(1"→ 6')-β-D-glucopyranoside(4),bis(2-ethylhexyl)benzene-1,2-dicarboxylate(5),(Z)-10-heneicosenoic acid(6),di-O-methylcrenati(7),methyl-α-D-fructofuranoside(8),β-daucosterol(9),syringic acid(10),vanillicacid(11),octacosanol(12),isoarborinol(13),2,7-dihydroxy-6-methyl-4-(1-methylethyl)-1-naphthalenecarboxylate(14),vanillin(15),coniferyl aldehyde(16),9(11)-dehydroergosterolperoxide(17),5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol(18),β-sitosterol(19),respectively.Compounds 1 and 2 were new compounds,compounds 5-11,13,15-18 were isolated from Alangium for the first time.The anti-inflammatory activity of compourd 1 was determinded by the LPS-induced RAW264.7 macrophage inflammation model.The results showed that the new compound 1 has a certain inhibitory effect on LPS-induced NO production of RAW264.7 cells,and the inhibitory rate was 54.57％.
基金项目
国家自然科学基金(U1812403)
贵州省高层次创新型人才培养项目([2016]5677)
维普
万方数据