Chemical constituents of 70％ethanol extract of Alangium chinense subsp.pauciflorum were investigated.The 70％ethanol extract of A.chinense subsp.pauciflorum was isolated and purified by D-101 macroporous resins,silica gel,Sephadex LH-20 and other methods.As a result,nineteen compounds were isolated and identified as 4-cyclohexene-1α,2α,3α-triol-1-O-β-D-glucoside(1),1β,4α,6α,13-tetrahydroxy-eudesm-11(12)-ene(2),sucrose(3),1'-O-benzyl-α-L-rhamnopyranosyl-(1"→ 6')-β-D-glucopyranoside(4),bis(2-ethylhexyl)benzene-1,2-dicarboxylate(5),(Z)-10-heneicosenoic acid(6),di-O-methylcrenati(7),methyl-α-D-fructofuranoside(8),β-daucosterol(9),syringic acid(10),vanillicacid(11),octacosanol(12),isoarborinol(13),2,7-dihydroxy-6-methyl-4-(1-methylethyl)-1-naphthalenecarboxylate(14),vanillin(15),coniferyl aldehyde(16),9(11)-dehydroergosterolperoxide(17),5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol(18),β-sitosterol(19),respectively.Compounds 1 and 2 were new compounds,compounds 5-11,13,15-18 were isolated from Alangium for the first time.The anti-inflammatory activity of compourd 1 was determinded by the LPS-induced RAW264.7 macrophage inflammation model.The results showed that the new compound 1 has a certain inhibitory effect on LPS-induced NO production of RAW264.7 cells,and the inhibitory rate was 54.57％.
Alangium chinense subsp.pauciflorumchemical constituentisolation and identification
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