摘要
黄芩提取物是舒肝宁注射液中重要的组分之一.该研究建立了一种超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定舒肝宁注射液及方中黄芩提取物的5种成分在大鼠胆汁、尿液和粪便中含量的方法,揭示舒肝宁注射液与单味黄芩提取物在大鼠体内的排泄过程差异,探讨黄苓配伍前后5种成分的体内排泄过程规律.大鼠分别尾静脉注射4.2 mL·kg-1的舒肝宁注射液和黄芩提取物,观察48 h内大鼠胆汁、尿液和粪便中黄芩苷、黄芩素、千层纸素A、千层纸素A-7-O-β-D-葡萄糖醛酸苷和野黄芩苷的动态排泄情况.结果显示,大鼠静脉注射舒肝宁注射液和黄芩提取物后,除黄芩苷外,其余4种指标成分均以较高的比例以原型成分排泄.各成分在尿液中排泄量较大,在粪便和胆汁中的排泄量较少.黄芩提取物经配伍后,5种指标成分在大鼠体内的累积排泄量均降低.其中,黄苓素在胆汁、尿液和粪便的累积排泄量显著降低了26.67％、48.11％、31.01％;黄芩苷在胆汁、尿液和粪便的累积排泄量显著降低了70.69％、19.43％、31.22％.研究表明,黄芩提取物中的5种指标成分主要经肾脏排泄,且注射液中其他组分可能会延缓其排泄过程,延长在体内的滞留时间.该研究对阐明舒肝宁注射液配伍合理性具有重要的意义.
Abstract
Scutellariae Radix extract is one of the important components in Shuganning Injection.In this study,an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was established for simultaneously determining five components in Shuganning Injection and Scutellariae Radix extract in bile,urine,and feces of rats,so as to reveal the difference in the excretion process of Shuganning Injection and Scutellariae Radix extract in rats and explore the law of the excretion process of the five components in vivo before and after the compatibility of Scutellariae Radix.Rats were injected with Shuganning Injection and Scutellariae Radix extract(4.2 mL·kg-1),respectively,and the excretion of baicalin,baicalein,oroxylin A,oroxylin A-7-O-β-D-glucuronide,and scutellarin in bile,urine,and feces of rats in 24 h was observed.The results showed that except for baicalin,the other four index components were excreted as prototype components in a high proportion after intravenous injection of Shuganning Injection and Scutellariae Radix extract in rats,respectively.The excretion of each component was relatively high in urine and less in feces and bile.After the compatibility of Scutellariae Radix extract,the accumulative excretion of five index components in rats all decreased.Among them,the cumulative excretion of baicalein in bile,urine,and feces significantly decreased by 26.67％,48.11％,and 31.01％.The cumulative excretion of baicalin in bile,urine,and feces decreased significantly by 70.69％,19.43％,and 31.22％.The result showed that the five index components in Scutellariae Radix extract were mainly excreted by the kidneys,and other components in Shuganning Injection delayed the excretion process and prolonged the residence time.This study is of great significance for elucidating the compatibility rationality of Shuganning Injection.
基金项目
贵州省科技计划(黔科合成果[2022]013)
贵州省科技计划(黔科合支撑[2023]—般158)
贵州省科技计划(黔科合中引地[2023]006)
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2022]031-1)
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]036)
维普
万方数据