摘要
对乌药醇提液的水部位化学成分进行研究,运用大孔树脂柱色谱、MCI中压柱色谱、半制备型高效液相色谱等方法进行分离纯化,并结合化合物的理化性质和波谱学数据分析鉴定化合物结构,从乌药醇提液的水部位中分离并鉴定了13个化合物,分别为(1S,3R,5R,6R,8S,10S)-epi-lindenanolide H(1)、它乔糖苷(2)、lindenanolide H(3)、leonuriside A(4)、3,4-二羟基苯乙醇葡萄糖苷(5)、3,4,5-三甲氧基苯酚-1-O-6-α-L-鼠李糖-(1→6)-O-β-D-葡萄糖苷(6)、5-羟甲基糠醛(7)、(+)-南烛木树脂-4-烯基-β-D-吡喃葡萄糖苷(8)、南烛木糖苷(9)、去甲波尔定碱(10)、去甲异波尔定碱(11)、波尔定碱(12)、瑞枯灵(13).其中化合物1为新化合物,化合物2、5、6、8、9为首次从乌药中分离得到.利用脂多糖(LPS)诱导RAW264.7细胞建立炎症模型,结果表明化合物1、8、10、12具有较好的抗炎活性.
Abstract
The chemical composition of the aqueous part of the extract from Lindera aggregata was studied,which was separated and purified by the macroporous resin column chromatography,MCI medium pressure column chromatography,semi-preparative high-performance liquid phase and other methods.The structures of the compounds were identified according to physical and chemical properties and spectroscopic data.Thirteen compounds were isolated and identified from the aqueous extracts,which were identified as(1S,3R,5R,6R,8S,10S)-epi-lindenanolide H(1),tachioside(2),lindenanolide H(3),leonuriside A(4),3,4-dihydroxyphenyl ethyl β-D-glucopyranoside(5),3,4,5-trimethoxyphenol-1-0-6-α-L-rhamnose-(1→6)-O-β-D-glucoside(6),5-hydroxymethylfurfural(7),(+)-lyoniresin-4-yl-β-D-glucopyranoside(8),lyoniside(9),norboldine(1O),norisopordine(11),boldine(12),reticuline(13).Among them,compound 1 was a new one,and compounds 2,5,6,8,9 were obtained from L.aggregata for the first time.The inflammatory model was induced by lipopolysaccharide(LPS)in the RAW264.7 cells.The results showed that compounds 1,8,10 and 12 had significant anti-inflammatory activity.
基金项目
山东省重大科技创新工程项目(2021CXGC010508)
山东省泰山学者项目()
齐鲁工业大学(山东省科学院)科教产项目(2022JBZ02-04)
NETL
NSTL
万方数据