Analysis of constituents in different parts of Forsythia suspensa by UPLC-Q-TOF-MS and evaluation of their anti-inflammatory activity
This study aims to characterize and identify the chemical constituents in 11 parts of Forsythia suspensa by using ultra-performance liquid chromatography-quadrupole time of flight-mass spectrometry(UPLC-Q-TOF-MS)combined with a self-established chemical constituent database,including leaves,flowers,fruits,green F.suspensa,old F.suspensa,and seeds.The quality attributes and differences of different parts of F.suspensa were evaluated by principal component analysis,partial least square discriminant analysis,and other stoichiometric methods.A total of 79 compounds were identified,including 13 phenylethanol glycosides,10 lignans,12 flavonoids,10 organic acids,14 terpenoids,and 20 other types of compounds.Among them,34 compounds were the main variables of difference between the different parts of F.suspensa,and the content of each component was relatively higher in the leaves and green F.suspensa.The LPS-induced inflammation model of RAW264.7 cells was applied to study the anti-inflammatory activity of the extracts of the different parts of F.suspensa and the main constituents.The results show that the extracts of green F.suspensa,flower,twig,and stem exhibited anti-inflammatory activity,and the constituents such as forsythoside A,phyllyrin,phillygenin,and(+)-pinoresinol-β-D-glucopyranoside could significantly inhibit anti-inflammatory activity released by NO.The chemical constituent in different parts of F.suspensa is analyzed comprehensively,and the anti-inflammatory activity is evaluated in this study,which provides a reference for the development and comprehensive utilization of F.suspensa resources.
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