摘要
建立淫羊藿Epimedium brevieornu中13种化学成分新绿原酸、绿原酸、隐绿原酸、木兰花碱、金丝桃苷、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅱ、箭藿苷A、淫羊藿次苷Ⅰ、宝藿苷Ⅰ的一测多评法,考察该方法在评价不同产地和不同品种的淫羊藿药材质量中具有一定的可行性和准确性.通过对实验方法的科学性及准确性考察,采用外标法测定淫羊藿中13种化学成分的含量,同时,以淫羊藿苷为内标,分别建立淫羊藿苷与新绿原酸、绿原酸、隐绿原酸、木兰花碱、金丝桃苷、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅱ、箭藿苷A、淫羊藿次苷Ⅰ、宝藿苷Ⅰ的相对较正因子,计算淫羊藿中新绿原酸、绿原酸、隐绿原酸、木兰花碱、金丝桃苷、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅱ、箭藿苷A、淫羊藿次苷Ⅰ、宝藿苷Ⅰ的含量,实现用一测多评法测定其含量,最后比较实测值与计算值的差异,来验证一测多评法在测定中的准确性及科学性.各成分相对校正因子重复性较好,外标法测定结果与一测多评测定结果无显著性差异.以淫羊藿苷为内标,同时测定新绿原酸、绿原酸、隐绿原酸、木兰花碱、金丝桃苷、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅱ、箭藿苷A、淫羊藿次苷Ⅰ、宝藿苷Ⅰ的一测多评法可用于淫羊藿的定量分析.
Abstract
The quantitative analysis of multicomponents by single-marker(QAMS)was established for 13 chemical components of Epimedii Folium,including neoglycolic acid,chlorogenic acid,cryo-chlorogenic acid,magnolidine,hypericin,epimedin A,epimedin B,epimedin C,icariin,baohuoside Ⅱ,sagittatoside A,icariin subside Ⅰ,and baohuoside Ⅰ,so as to investigate the feasibility and accuracy of this method in evaluating the quality of Epimedii Folium materials from different origins and different varieties.Through the scientific and accurate investigation of the experimental method,the external standard method was used to determine the content of 13 chemical components in epimedium brevieornu.At the same time,icariin was used as the internal standard,and the relative correction factors of icariin with neoglycolic acid,chlorogenic acid,cryo-chlorogenic acid,magnolidine,hypericin,epimedin A,epimedin B,epimedin C,icariin,baohuoside Ⅱ,sagittatoside A,icariin subside Ⅰ,and baohuoside Ⅰ were established,respectively.The contens of neoglycolic acid,chlorogenic acid,cryo-chlorogenic acid,magnolidine,hypericin,epimedin A,epimedin B,epimedin C,icariin,baohuoside Ⅱ,sagittatoside A,icariin subside Ⅰ,and baohuoside Ⅰ in Epimedii Folium were calculated by QAMS.Finally,the difference between the measured value and the calculated value was compared to verify the accuracy and scientific nature of QAMS in the determination.The relative correction factor of each component had better repeatability,and there was no significant difference between the results of the external standard method and those of QAMS.With icariin as the internal standard,QAMS simultaneously determining neoglycolic acid,chlorogenic acid,cryo-chlorogenic acid,magnolidine,hypericin,epimedin A,epimedin B,epimedin C,icariin,baohuoside Ⅱ,sagittatoside A,icariin subside Ⅰ,and baohuoside Ⅰ can be used for quantitative analysis of Epimedii Folium.
基金项目
甘肃省药品监督管理局科技项目(2021GSMPA-KL01)
甘肃省药品监督管理局科技项目(2021GSMPA-KL02)
NETL
NSTL
万方数据