Ginsenoside Re regulates mitochondrial biogenesis through Nrf2/HO-1/PGC-1αpathway to reduce hypoxia/reoxygenation injury in H9c2 cells
This article explored the mechanism by which ginsenoside Re reduces hypoxia/reoxygenation(H/R)injury in H9c2 cells by regulating mitochondrial biogenesis through nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)/peroxisome prolife-rator-activated receptor gamma coactivator-1α(PGC-1α)pathway.In this study,H9c2 cells were cultured in hypoxia for 4 hours and then reoxygenated for 2 hours to construct a cardiomyocyte H/R injury model.After ginsenoside Re pre-administration intervention,cell activity,superoxide dismutase(SOD)activity,malondialdehyde(MDA)content,intracellular reactive oxygen species(Cyto-ROS),and intramitochondrial reactive oxygen species(Mito-ROS)levels were detected to evaluate the protective effect of ginsenoside Re on H/R injury of H9c2 cells by resisting oxidative stress.Secondly,fluorescent probes were used to detect changes in mitochondrial membrane potential(△Ψm)and mitochondrial membrane permeability open pore(mPTP),and immunofluorescence was used to detect the expression level of TOM20 to study the protective effect of ginsenoside Re on mitochondria.Western blot was further used to detect the protein expression levels of caspase-3,cleaved caspase-3,Cyto C,Nrf2,HO-1,and PGC-1α to explore the specific mechanism by which ginsenoside Re protected mitochondria against oxidative stress and reduced H/R injury.Compared with the model group,ginse-noside Re effectively reduced the H/R injury oxidative stress response of H9c2 cells,increased SOD activity,reduced MDA content,and decreased Cyto-ROS and Mito-ROS levels in cells.Ginsenoside Re showed a good protective effect on mitochondria by increasing △Ψm,reducing mPTP,and increasing TOM20 expression.Further studies showed that ginsenoside Re promoted the expression of Nrf2,HO-1,and PGC-1α proteins,and reduced the activation of the apoptosis-related regulatory factor caspase-3 to cleaved caspase-3 and the expression of Cyto C protein.In summary,ginsenoside Re can significantly reduce I/R injury in H9c2 cells.The specific mechanism is related to the promotion of mitochondrial biogenesis through the Nrf2/HO-1/PGC-1α pathway,thereby increasing the number of mitochondria,improving mitochondrial function,enhancing the ability of cells to resist oxidative stress,and alleviating cell apoptosis.
NETL
NSTL
万方数据
