Bupleuri Radix-Paeoniae Radix Alba medicated plasma exerts effects on HepG2 hepatoma cells by regulating miR-1297/PTEN signaling axis
The present study aimed to investigate the effect and mechanism of Bupleuri Radix-Paeoniae Radix Alba medicated plasma on HepG2 hepatoma cells by regulating the microRNA-1297(miR-1297)/phosphatase and tensin homologue deleted on chromosome 10(PTEN)signaling axis.Real-time quantitative PCR(RT-qPCR)was carried out to determine the mRNA levels of miR-1297 and PTEN in different hepatoma cell lines.The dual luciferase reporter assay was employed to verify the targeted interaction between miR-1297 and PTEN.The cell counting kit-8(CCK-8)was used to detect cell proliferation,and the optimal concentration and intervention time of the medicated plasma were determined.The cell invasion and migration were examined by Transwell assay and wound healing assay.Cell cycle distribution was detected by PI staining,and the apoptosis of cells was detected by Annexin V-FITC/PI double staining.The mRNA levels of miR-1297,PTEN,protein kinase B(Akt),and phosphatidylinositol 3-kinase(PI3K)were determined by RT-qPCR.Western blot was employed to determine the protein levels of PTEN,Akt,p-Akt,caspase-3,caspase-9,B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X protein(Bax).The results showed that HepG2 cells were the best cell line for subsequent experiments.The dual luciferase reporter assay confirmed that miR-1297 could bind to the 3'-untranslated region(3'UTR)in the mRNA of PTEN.The medicated plasma inhibited the proliferation of HepG2 cells,and the optimal intervention concentration and time were 20％and 72 h.Compared with the blank plasma,the Bupleuri Radix-Paeoniae Radix Alba medicated plasma,miR-1297 inhibitor,miR-1297 inhibitor+medicated plasma all inhibited the proliferation,invasion,and migration of HepG2 cells,increased the proportion of cells in the G0/G1 phase,decreased the proportion of cells in the S phase,and increased the apoptosis rate.The medicated plasma down-regulated the mRNA levels of miR-1297,PI3K,and Akt and up-regulated the mRNA level of PTEN.In addition,it up-regulated the protein levels of PTEN,Bax,caspase-3,and caspsae-9 and down-regulated the protein levels of p-Akt,p-PI3K,and Bcl-2.In conclusion,Bupleuri Radix-Paeoniae Radix Alba medicated plasma can inhibit the expression of miR-1297 in HepG2 hepatoma cells,promote the expression of PTEN,and negatively regulate PI3K/Akt signaling pathway,thereby inhibiting the proliferation and inducing the apoptosis of HepG2 cells.
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