运用宏基因组技术研究施加中药渣有机肥对丹参及土壤微生物群落的影响
Effects of organic fertilizer from traditional Chinese medicine residues on growth and soil microbial community of Salvia miltiorrhiza by metagenomic technique
王紫涵 1刘莎 1杨光 1陆祖瑜 1朱荣清 1李颖 1申业 1康利平 1陈美兰1
作者信息
- 1. 中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室,北京 100700
- 折叠
摘要
土壤微生物组是土壤健康的关键性评价指标,前期的研究表明中药渣有机肥能够提高栽培中药材的产量和质量,但是中药渣有机肥对于土壤微生物组的影响相关的报道很少.基于此该研究在评价中药渣有机肥对栽培丹参产量和质量影响的基础上,采用宏基因组测序技术研究中药渣有机肥对栽培丹参根际微生物组群落以及功能的影响.实验结果表明①施用中药渣有机肥促进了丹参生长以及有效成分的积累,其中丹参地上干重、地下干重、地上鲜重、地下鲜重的提高幅度为371.4％、288.3％、313.4％、151.9％;迷迭香酸和丹酚酸B的提高幅度为887.0％、183.0％.②施用中药渣有机肥显著改变了栽培丹参根际细菌和真菌群落结构,微生物的群落组成具有显著性的区别.③施用中药渣有机肥能够显著提高多亚硝化螺菌 Nitrosospira multiformis、枯草芽孢杆菌 Bacillus subtilis、产酶溶杆菌 Lysobacter enzymogenes 以及木霉菌 Trichoderma 等土壤有益菌的相对丰度.④KEGG功能预测分析表明,施加有机肥后的土壤环境中更容易富集与代谢相关的微生物;施加中药渣有机肥后还可增加与硝化作用、反硝化作用相关的功能基因的丰度.该研究结果为今后中药渣有机肥在中药材栽培中的应用提供指导,丰富了中药材绿色栽培技术的内容.
Abstract
Soil microbiome is a key evaluation index of soil health.Previous studies have shown that organic fertilizer from tradi-tional Chinese medicine(TCM)residues can improve the yield and quality of cultivated traditional Chinese medicinal materials.However,there are few reports on the effects of organic fertilizer from TCM residues on soil microbiome.Therefore,on the basis of evaluating the effects of organic fertilizer from TCM residues on the yield and quality of cultivated Salvia miltiorrhiza,the metagenomic sequencing technique was used to study the effects of organic fertilizer from TCM residues on rhizosphere microbiome community and function of cultivated S.miltiorrhiza.The results showed that:① the application of organic fertilizer from TCM residues promoted the growth of S.miltiorrhiza and the accumulation of active components,and the above-ground and underground dry weight and fresh weight of S.miltiorrhiza increased by 371.4％,288.3％,313.4％,and 151.9％.The increases of rosmarinic acid and salvianolic acid B were 887.0％and 183.0％.②The application of organic fertilizer from TCM residues significantly changed the rhizosphere bac-terial and fungal community structures,and the microbial community composition was significantly different.③The relative abundance of soil-beneficial bacteria,such as Nitrosospira multiformis,Bacillus subtilis,Lysobacter enzymogenes,and Trichoderma was significant-ly increased by the application of organic fertilizer from TCM residues.④KEGG function prediction analysis showed that metabolism-related microorganisms were more easily enriched in the soil environment after organic fertilizer application.The abundance of function-al genes related to nitrification and denitrification could also be increased after the application of organic fertilizer from TCM residues.The results of this study provide guidance for the future application of organic fertilizer from TCM residues in the cultivation of traditio-nal Chinese medicinal materials and enrich the content of green cultivation technology of traditional Chinese medicinal materials.
关键词
丹参/中药渣有机肥/土壤微生物/群落结构多样性/宏基因组Key words
Salvia miltiorrhiza/organic fertilizer from traditional Chinese medicine residues/soil microorganism/community struc-ture diversity/metagenome引用本文复制引用
基金项目
中国中医科学院科技创新工程项目(CI2021A03906)
国家自然科学基金面上项目(82173931)
出版年
2024
NETL
NSTL
万方数据