基于非靶向代谢组学研究不同炮制方法郁李仁对于小鼠肝脾代谢调节作用
Non-targeted metabolomics of spleen and liver metabolism in mice treated with Pruni Semen processed with different methods
贺佳诺 1张阳 2郑雨佳 2徐海峰 1李宇健 1米婧璇 1赵雯雅 2杨雪 2郝光飞 3赵山山 3刘洪伟2
作者信息
- 1. 河北工程大学生命科学与食品工程学院,河北邯郸 056038;河北省科学院生物研究所,河北石家庄 050081
- 2. 河北省科学院生物研究所,河北石家庄 050081
- 3. 河北工程大学生命科学与食品工程学院,河北邯郸 056038
- 折叠
摘要
利用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS)开展生制和炒制2种炮制方法处理的郁李仁对于小鼠肝脾代谢调节影响的研究,旨在通过代谢组学解析不同炮制方法郁李仁对于机体的分子作用机制.将24只雄性小鼠随机分为生制组、炒制组和对照组,以0.01 g·mL-1的郁李仁水煎液经口给药1周.给药结束后取肝脏和脾脏组织,采用液相色谱质谱法(LC-MS)进行代谢组学分析,以揭示郁李仁对小鼠肝脾代谢影响.结果显示,生制组小鼠脾脏中11种差异代谢物的表达量显著上调(P＜0.05),57种代谢物表达量显著下调(P＜0.05);肝脏中15种差异代谢物表达量显著上调(P＜0.05),58种代谢物显著下调(P＜0.05).炒制组小鼠脾脏中31种差异代谢物表达量显著上调(P＜0.05),10种代谢物显著下调(P＜0.05);肝脏中26种差异代谢物表达量显著上调(P＜0.05),61种代谢物显著下调(P＜0.05).生制组肝脾中的代谢物主要涉及丙氨酸、天冬氨酸和谷氨酸代谢,嘌呤代谢,氨基糖和核苷酸糖代谢,D-谷氨酰胺和D-谷氨酸代谢;炒制组肝脾中的代谢物主要涉及核黄素代谢,氨基糖和核苷酸糖代谢,嘌呤代谢,丙氨酸、天冬氨酸和谷氨酸代谢,D-谷氨酰胺和D-谷氨酸代谢,谷胱甘肽代谢.由此推测,2种炮制方法处理的郁李仁均会通过影响谷氨酰胺和谷氨酸代谢进而对小鼠肝脾代谢产生影响.
Abstract
Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was employed to investigate the impacts of Pruni Semen processed with different methods(raw and fried)on the liver and spleen metabolism in mice.A total of 24 male mice were randomly assigned to three groups:raw Pruni Semen group,fried Pruni Semen group,and control(deionized water)group.Mice in the three groups were orally administrated with 0.01 g·mL-1 Pruni Semen decoction or deionized water for one week.After that,the liver and spleen tissues were collected,and liquid chromatography-mass spectrometry(LC-MS)-based metabolomic analysis was carried out to investigate the impact of Pruni Semen on the liver and spleen metabolism in mice.Compared with thte control group,the raw Pruni Semen group showed up-regulation of 11 metabolites and down-regulation of 57 metabolites in the spleen(P＜0.05),as well as up-regulation of 15 metabolites and down-regulation of 58 metabolites in the liver(P＜0.05).The fried Pruni Semen group showed up-regulation of 31 metabolites and down-regulation of 10 metabolites in the spleen(P＜0.05),along with up-regulation of 26 metabolites and down-regulation of 61 metabolites in the liver(P＜0.05).The differential metabolites identified in the raw Pruni Semen group were primarily associated with alanine,aspartate,and glutamate metabolism,purine metabolism,amino sugar and nucleotide sugar metabolism,and D-glutamine and D-glutamate metabolism.The differential metabolites identified in the fried Pruni Semen group predominantly involved riboflavin metabolism,amino sugar and nucleotide sugar metabolism,purine metabolism,alanine,aspartate,and glutamate metabolism,D-glutamine and D-glutamate metabolism,and glutathione metabolism.The findings suggest that both raw and fried Pruni Semen have the potential to modulate the metabolism of the liver and spleen in mice by influencing the glutamine and glutamate metabolism.
关键词
郁李仁/代谢组学/肝脏/脾脏/差异代谢物/差异代谢通路Key words
Pruni Semen/metabolomics/liver/spleen/differential metabolites/differential metabolic pathway引用本文复制引用
基金项目
河北省科学院基本科研业务费项目(2023PF02)
河北省科学院科技项目(23312)
河北省科学院高层次人才培养与资助项目(2022G01)
出版年
2024
NETL
NSTL
万方数据