滇鼠刺中1个新的降新木脂素类化合物
A new nor-neolignan compound identified from Itea yunnanensis
简进珍 1郎天琼 1张伟 1封云倩 1罗国勇1
作者信息
- 1. 贵州中医药大学 药学院, 贵州 贵安 550025
- 折叠
摘要
采用多种分离手段,并结合波谱数据分析、X-射线单晶衍射分析和文献数据对比等方法,对滇鼠刺Itea yunnanensis的化学成分进行研究,从中分离并鉴定了 7 个化合物,分别为(S)-3-[1-(4-羟基苯基)丙烷-2-基]-4-甲氧基苯甲酸甲酯(1)、iteafuranal B(2)、syringaresinol(3)、二氢山柰酚(4)、均三甲氧基苯(5)、正二十烷(6)、正二十九烷(7).其中,化合物 1 为新的降新木脂素类化合物,命名为iteanorneoligan A;其余化合物均为首次从该植物中分离得到.基于Sk-hep-1 细胞模型,采用MTT法评价化合物的抗肝癌活性,发现化合物 2 对Sk-hep-1 细胞表现出显著增殖抑制活性,IC50 为 9.4 μmol·L-1.采用DP-PH、ABTS·+和O2(-·)的自由基清除活性评价化合物的抗氧化潜力,发现化合物1表现出显著的ABTS·+自由基清除活性,IC50为0.178 mg·mL-1.
Abstract
Various separation methods in combination with spectral data analysis,X-ray single crystal diffraction analysis,and litera-ture data comparison were employed to clarify the chemical constituents of Itea yunnanensis.Seven compounds were obtained from I.yunnanensis,which were identified as(S)-3-[1-(4-hydroxyphenyl)propane-2-yl]-4-methoxybenzoate methyl ester(1),iteafuranal B(2),syringaresinol(3),dihydrokaempferol(4),trimethoxybenzene(5),eicosane(6),and nonacosane(7),respectively.Among them,compound 1 was a new nor-neolignan compound named iteanorneoligan A,and the rest of the compounds were identified from I.yunnanensis for the first time.The anti-hepatocellular carcinoma effect of the compound was evaluated based on Sk-hep-1 cells model via MTT assay,and compound 2 showed a significant inhibitory effect on the proliferation of Sk-hep-1 cells with an IC50 of 9.4 μmol·L-1.The antioxidant capacity was determined via DPPH,ABTS·+,and O2(-·)radical scavenging ability,and compound 1 ex-hibited a significant ABTS·+ radical scavenging effect with an IC50 of 0.178 mg·mL-1.
关键词
滇鼠刺/降新木脂素/iteanorneoligan/A/抗肝癌/抗氧化Key words
Itea yunnanensis/nor-neolignan/iteanorneoligan A/anti-hepatocellular carcinoma effect/anti-oxidant引用本文复制引用
基金项目
贵州省"千层次"创新人才项目(贵中医[ZQ2018002])
国家自然科学基金项目(81860764)
贵州省高层次人才创新创业择优项目(高层次人才择优资助合同202107号)
贵阳中医学院 2018年度学术新苗培养及创新探索专项(黔科合平台人才[2017]5735号-23)
出版年
2024
NETL
NSTL
万方数据