Chemical constituents from the ethanol extract of Picrorhiza scrophulariiflora were isolated and purified by column chromatography.Their structures were identified by HR-MS,1D and 2D-NMR,and their cytotoxicity was assessed by CCK-8 assay.Four compounds were isolated and identified as follows:2β-D-glucosyloxy-3/3,16α,20β-trihydroxy-9-methyl-19-norlanosterol-5,25-diene-22-one(1),2β-D-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,24-diene-22-one(2),25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5-ene-22-one(3)and 25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,23-(E)-diene-22-one(4).Compound 1 represents a new cucurbitane glycoside.The half inhibitory concentrations of the 4 compounds exceeded 100 μmol·L-1 against four tumor cell lines,indicating no significant cytotoxicity.
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