Pseudo-targeted metabolomics for differential components of Rhei Radix et Rhizoma from three plant species
Rhei Radix et Rhizoma is common traditional Chinese medicine with multiple original plants.The content and proportion of the active components in Rhei Radix et Rhizoma from different plant species were compared to accurately evaluate the medicine qua-lity and provide a theoretical basis for precise use of this medicine in clinical practice.In this study,fresh Rhei Radix et Rhizoma sam-ples were collected from the four-year-old plants of Rheum palmatum,R.tanguticum,and R.officinale.The relative content of 220 anthraquinones,anthrones,and tannins in the samples were determined by pseudo-targeted metabolomics,and the differential compo-nents were screened by multivariate statistical methods.The principal component analysis classified the samples into three clusters ac-cording to the original plants.The orthogonal partial least squares-discriminant analysis(OPLS-DA)screened out 117 differential com-ponents,including 8 free anthraquinones,18 anthraquinone glycosides,80 anthrones,and 11 tannins.Twenty-eight components had the highest content in R.tanguticum,mainly including sennosides,anthraquinone glycosides,and procyanidins.Thirty-five compo-nents showed the highest content in R.officinale,mainly including free anthraquinones and catechines.Fifty-four components showed the highest content in R.palmatum,mainly including dianthrones,while the structures of most of them cannot be determined tempora-rily.The content distribution of differential components in the three original plants indicates that R.tanguticum has the strongest effect of purging,while R.officinale has the strongest effect of clearing heat and purging fire,and both have stronger effects of resolvong sta-sis and dredging meridians than R.palmatum.
Rhei Radix et Rhizomamultiple original plantspseudo-targeted metabolomicsdifferential componentsprecise Chi-nese medicine
NETL
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万方数据
