川贝母HSP70家族基因鉴定及高温胁迫下不同组织的表达分析
Identification of HSP70 gene family members in Fritillaria cirrhosa and expression analysis in different tissues under high temperature stress
张晓玉 1余济辰 1陈文涛 1周芏延 1袁媛 1刘鸿高 2梁艳丽1
作者信息
- 1. 云南农业大学 西南中药材种质创新与利用国家地方联合工程研究中心,云南 昆明 650201;云南农业大学 云南省药用植物生物学重点实验室,云南 昆明 650201;云南农业大学 农学与生物技术学院,云南 昆明 650201
- 2. 云南农业大学 农学与生物技术学院,云南 昆明 650201
- 折叠
摘要
热激蛋白 70(heat shock protein 70,HSP70)是植物响应逆境胁迫的一类应激蛋白,在生物体内分布广,具有高度保守性.为解析川贝母响应高温胁迫的分子机制,该研究利用川贝母根、鳞茎、茎、叶、果不同组织的转录组数据,通过生物信息学方法对HSP70 家族基因进行鉴定与筛选,并采用qRT-PCR技术分析其在高温(35℃)胁迫下的表达模式.结果显示,共鉴定出 26 个川贝母HSP70 成员,分别命名为FcHSP70-1～FcHSP70-26,编码蛋白的氨基酸数量为 560～944 个,相对分子质量为61.64～100.01 kDa,理论等电点为 5.00～6.59,蛋白质二级以无规卷曲和α-螺旋为主.亚细胞定位预测分析表明,FcHSP70s在线粒体、叶绿体、细胞核、内质网和细胞质中均有分布.系统进化树分析表明,26 个川贝母FcHSP70s中 7 个属于Dnak亚家族,19 个属于HSP110/SSE亚家族.在 35℃处理下,FcHSP70-5、FcHSP70-8、FcHSP70-17、FcHSP70-18 和FcHSP70-23 在川贝母5 个组织中的表达量均显著上升,最高上调倍数为 156.54,推测在川贝母植株响应高温胁迫中起主要作用.另外相较于其他组织,茎和叶片对高温最为敏感,在 18 个基因中的平均上调倍数分别为 18.18、8.03,说明川贝母主要感受高温的组织可能是茎和叶片.该研究为深入解析HSP70 家族基因在川贝母高温胁迫中的功能奠定基础.
Abstract
The heat shock protein 70 family contains the stress proteins ubiquitous in plants.These proteins are involved in the re-sponses to different abiotic stress conditions and have highly conserved gene sequences.However,little is known about the molecular mechanisms of Fritillaria cirrhosa in response to high-temperature stress.Here,26 HSP70s,FcHSP70-1 to FcHSP70-26,were identi-fied from the transcriptome data of root,bulb,stem,leaf,and fruit samples of F.cirrhosa.The proteins encoded by FcHSP70s had the lengths ranging from 560 aa to 944 aa,with the molecular weight of 61.64-100.01 kDa and the theoretical isoelectric point between 5.00 and 6.59.The secondary structural elements of HSP70s were mainly random coils and α-helixes.Subcellular localization predic-tion revealed that FcHSP70s were distributed in mitochondria,chloroplasts,nuclei,endoplasmic reticulum,and cytoplasm.The phylo-genetic tree showed that 7 members of the HSP70 family belonged to the Dnak subfamily and 19 members belonged to the HSP110/SSE subfamily.In addition,the qRT-PCR results showed that the expression of FcHSP70-5,FcHSP70-8,FcHSP70-17,FcHSP70-18,and FcHSP70-23 in F.cirrhosa was significantly up-regulated at 35℃,which indicated that these genes might play a role in the response to high temperature stress.In addition,compared with other tissues,stems and leaves were sensitive to high temperature stress,with the expression of 18 genes up-regulated by 18.18 and 8.03 folds on average,respectively.These findings provide valuable information about the molecular mechanism of HSP70s of F.cirrhosa in response to high temperature stress.
关键词
川贝母/HSP70/高温胁迫/生物信息学Key words
Fritillaria cirrhosa/HSP70/high temperature stress/bioinformatics引用本文复制引用
基金项目
云南省科技厅科技重大专项(202302AA310030)
迪庆州科技计划(2023ZXND01)
云南省科技计划(202102AA310031)
云南省科技厅乡村振兴科技专项(202304BI090009)
云南省重大专项(202102AA310045)
出版年
2024
NETL
NSTL
万方数据