Research on differential quality markers of medicine herbs for Danggui Buxue Decoction before and after processing based on fingerprint and network pharmacology
The different quality markers of Danggui Buxue Decoction before and after processing were studied based on fingerprint and network pharmacological research,and the seven screened index components were quantitatively analyzed,so as to provide an ex-perimental basis for the quality evaluation of Danggui Buxue Decoction before and after processing.HPLC method was used to establish fingerprints of Danggui Buxue Decoction before and after processing,and a multivariate statistical method was used to analyze the cha-racteristic maps and common peak areas of Danggui Buxue Decoction before and after processing.The different characteristic compo-nents before and after processing were screened out,and related targets and pathways of their different components were constructed based on network pharmacology.Their components were quantitatively analyzed.A total of 13 common peaks were identified in the fin-gerprint of the Danggui Buxue Decoction sample,and seven main chemical components were identified,with similarity>0.911.Fur-ther cluster analysis,principal component analysis,and partial least squares discriminant analysis were used to distinguish raw and pro-cessed products.According to VIP value,the main difference components 1,2,6,13,and 5 of Danggui Buxue Decoction before and after processing were screened.By combining the"five principles"of TCM Q-marker and network pharmacology,5-hydroxymethylfur-fural,ferulic acid,calycosin-7-O-β-D-glucoside,calycosin,ligusticolide,formononetin,and ligusticolide I were selected as the signa-ture components of quality difference before and after processing.The results of the quantitative analysis showed that the content of ligu-strin I,calycosin,formononetin,and ligusticum decreased after the Danggui Buxue Decoction was processed.The content of calycosin-7-O-β-D-glucoside and ferulic acid increased.At the same time,a new chemical compound,namely 5-hydroxymethylfurfural was pro-duced.The established fingerprint analysis method is stable and reliable.Combined with network pharmacology and quantitative re-search,it screens out the differential Q-marker,which provides an experimental basis for further research on processed products of Danggui Buxue Decoction.
NETL
NSTL
万方数据
