摘要
该研究选定紫丁香结香部位乙醇提取物,通过硅胶柱色谱、薄层板制备以及制备液相色谱等进行系统分离与纯化,并结合紫外分析(UV)、红外分析(IR)、核磁共振(NMR)、高分辨质谱(HR-ESI-MS)、X射线衍射等波谱技术及文献理化数据比较等方法进行结构鉴定,共鉴定出 10 个化合物,分别为紫丁香倍半萜D(1)、(-)-T-muurolol(2)、紫丁香倍半萜E～G(3～5)、14-noreudesma-3-hydroxy-3-en-2,9-dione(6)、1-isopropyl-2,7-dimethylnaphthalene(7)、异水菖蒲二醇(8)、α-二去氢菖蒲烯(9)、cadin-4-en-1-β-ol(10).其中化合物 1 为未见报道的倍半萜类新化合物,其余 9 个化合物均为首次从紫丁香中分离得到.化合物 1 对脂多糖(LPS)诱导的RAW264.7 细胞炎性损伤模型具有显著的保护作用.
Abstract
In this study,the chemical components of ethanol extract from the aromatic parts of Syringa oblata were systematically separated and purified by silica gel column chromatography,thin layer plate preparation and liquid phase preparation.Combined with ultraviolet analyzer(UV),infrared analyzer(IR),nuclear magnetic resonance analyzer(NMR),high resolution mass spectrometer(HR-ESI-MS),X-ray diffraction and other spectrum technology as well as literature physicochemical data comparison methods for structural identification,a total of 10 compounds were identified.They were identified as oblatanoid D(1),(-)-T-muurolol(2),oblatanoid E-G(3-5),14-noreudesma-3-hydroxy-3-en-2,9-dione(6),1-isopropyl-2,7-dimethylnaphthalene(7),isocoradiol(8),α-calacorene(9),cadin-4-en-1-β-ol(10).Compound 1 is a new sesquiterpene compound that has not been reported,and the other 9 compounds are isolated from S.oblata for the first time.The compound 1 has a significant protective effect on the LPS-induced inflam-matory injury model of RAW264.7 cells.
基金项目
内蒙古自治区自然科学基金青年基金(2022QN08028)
内蒙古自治区自然科学基金青年基金(2022LHQN08004)
中央本级重大增减支项目(2060302)
中国中医科学院科技创新工程项目(CI2021A041003)
内蒙古民族大学博士启动基金(BSZ012)
国家药品监督管理局中药(蒙药)质量控制重点实验室开放基金(MDK2023026)
NETL
NSTL
万方数据